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Testosterone, Serum (Total)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of testosterone, a key male sex hormone, in your blood. Testosterone plays a role in muscle and bone development, as well as sexual function and mood.

Quick summary

["Your testosterone levels help determine if you have healthy hormone balance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58810-3
Short code
Testost sp2 SerPl-mCnc
Synonyms
Testost sp2 SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order a total testosterone test to check for conditions affecting hormone levels, such as low testosterone (hypogonadism) or high testosterone (hyperandrogenism). It can also be used to evaluate symptoms like low libido, fatigue, or changes in body hair. Sometimes, it’s part of a broader evaluation for infertility, sleep disorders, or other health concerns linked to hormone imbalances.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if your blood is drawn early in the morning. Avoid smoking, caffeine, or strenuous activity for a few hours before your appointment. If you’re taking medications that affect hormones, let your healthcare provider know.

What the Test Involves

A healthcare professional will draw blood from a vein in your arm using a small needle. You may feel a slight pinch, but the procedure usually takes less than 5 minutes. The blood sample is then sent to a lab for analysis.

What the Results Mean

Testosterone levels vary by age, sex, and health status. Your results will be compared to reference ranges provided by your doctor, who will interpret them in the context of your overall health and symptoms. Abnormal results may prompt further testing or follow-up.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total testosterone

Frequently asked questions

What could cause abnormal results?
Low testosterone levels may be caused by conditions like pituitary disorders, kidney or liver disease, obesity, or aging. It can also result from medications (e.g., steroids), injuries, or genetic factors. High testosterone levels may occur with conditions like Cushing’s syndrome, polycystic ovary syndrome (PCOS), or tumors. Always discuss your symptoms and medical history with your doctor to understand potential causes.
How often should I get this test?
Your doctor will determine how often you need this test based on your health needs. For example, men with suspected low testosterone might get retested every 6–12 months, while others may need it only once. Regular check-ups or symptoms (like fatigue or mood changes) may guide when to repeat the test.
Can lifestyle changes affect testosterone levels?
Yes! Exercise, a balanced diet, stress management, and avoiding smoking or excessive alcohol can help maintain healthy testosterone levels. Weight management, particularly reducing excess body fat, is especially important since obesity is linked to lower testosterone in men.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy