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Total Serum Testosterone (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. Testosterone plays a role in muscle and bone development, red blood cell production, and sexual function.

Quick summary

["Your testosterone levels help determine if you have healthy hormone balance, especially important for men and women experiencing symptoms of low testosterone."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59993-6
Short code
Testost sp3 p chal SerPl-mCnc
Synonyms
Testost sp3 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like low libido, fatigue, or changes in mood and energy. It could also be part of evaluations for infertility, hormonal imbalances, or conditions like hypogonadism (low testosterone). Some men with prostate cancer may also have this test as part of monitoring.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if it involves drawing blood from a vein. Wear comfortable clothing and plan to stay near the testing site for a short time. If you’re taking medications that affect hormones, let your provider know.

What to Expect During the Test

A healthcare professional will insert a small needle into a vein in your arm to draw blood. You may feel a slight pinch, but it’s usually over quickly. The blood sample is then sent to a lab for analysis. The process typically takes about 15 minutes.

What the Results Mean

Results are compared to a reference range that varies by age, sex, and health status. Low testosterone levels may indicate conditions like hypogonadism, while very high levels could suggest hormonal disorders or other health issues. Your provider will discuss the results in the context of your overall health and symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Testosterone

Frequently asked questions

What Does My Testosterone Level Tell Me About My Health?
Testosterone levels can affect energy, mood, muscle strength, and sexual function. Low levels may cause fatigue, reduced muscle mass, or difficulty concentrating. High levels might not always be harmful but can sometimes signal underlying conditions like tumors or liver problems. Always discuss your results with your healthcare provider.
How Often Should I Get This Test?
The frequency depends on your symptoms, medical history, and age. For example, men over 40 may get baseline tests more often, while others might need monitoring if they have conditions like diabetes or obesity. Your provider will guide you on how often to retest.
Can I Take Anything Before the Test?
If fasting is required, avoid eating or drinking (except water) for the specified period. If you’re unsure, ask your provider. Some medications, like certain antidepressants or steroids, can affect testosterone levels, so let your provider know if you’re taking them.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy