Skip to content

Testosterone, Serum (Total)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of testosterone, a key male sex hormone, in your blood. Testosterone plays a role in muscle and bone development, red blood cell production, and sexual function.

Quick summary

["Your testosterone levels help assess male reproductive health and symptoms like low energy or sexual dysfunction."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59991-0
Short code
Testost sp5 p chal SerPl-mCnc
Synonyms
Testost sp5 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like low libido, erectile dysfunction, fatigue, or changes in body hair. It may also be checked during fertility evaluations, before surgery (e.g., prostate), or if you have conditions like hypogonadism (low testosterone) or Cushing’s syndrome.

What to Expect Before the Test

No special preparation is needed, but avoid smoking or drinking alcohol 24 hours before the draw. If you’re taking medications (e.g., steroids), let your provider know—some can affect testosterone levels.

What the Results Mean

Results help diagnose hormonal imbalances or conditions affecting testosterone production. Low levels may indicate issues with the pituitary gland, testes, or adrenal glands, while high levels could signal tumors or other disorders.

Common Conditions Linked to Testosterone Testing

Testosterone is often checked in men with suspected androgen deficiency, delayed puberty, or after injuries/medications that might disrupt hormone balance. Women may also have it tested if symptoms like irregular periods or hair loss suggest hormonal imbalances.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Testosterone’s Role in Health

Short name

Total Serum Testosterone

Frequently asked questions

How long does it take to get results after the test?
Results are typically available within 1–3 business days, depending on lab processing. Your provider will discuss them with you during a follow-up visit.
Can diet or exercise affect my testosterone levels?
Yes! Healthy fats, lean proteins, and regular exercise support hormone production. Avoid excessive alcohol and smoking, as they can lower testosterone. Stress and poor sleep may also impact levels.
What if my results are outside the expected range?
If results are abnormal, your provider will review them with you and may recommend further testing (e.g., free testosterone, LH/FSH levels) or treatments like hormone therapy if needed.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy