Skip to content

Testosterone, Serum (Total)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of testosterone, a key male sex hormone, in your blood. It can help diagnose hormonal imbalances, fertility issues, or conditions like low testosterone.

Quick summary

["Total testosterone levels help assess hormone health and potential hormone-related concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59988-6
Short code
Testost sp8 p chal SerPl-mCnc
Synonyms
Testost sp8 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like low energy, reduced sex drive, muscle weakness, or irregular menstrual cycles (in women). It may also be used to evaluate conditions such as hypogonadism (low testosterone), infertility, or to monitor treatment for hormone-related disorders.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the blood draw if your provider specifies it. Avoid heavy exercise or smoking for 24 hours before testing, as these can temporarily affect hormone levels.

How the Test Works

Blood is drawn from a vein in your arm, and the sample is sent to a lab where it is analyzed using an immunoassay method to measure total testosterone concentration.

What the Results Mean

Results are compared to general reference ranges, but individual needs vary. Your provider will interpret the results in the context of your symptoms, medical history, and other tests.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Serum Testosterone

Frequently asked questions

What causes testosterone levels to be tested?
Testosterone levels are often checked if you experience symptoms like fatigue, low libido, or changes in body hair/mood. It may also be part of routine evaluations for menopause, fertility issues, or chronic illnesses like obesity or diabetes.
How long does it take to get results?
Results typically arrive within 1–3 business days, depending on your lab’s processing time. Your provider will contact you once they are ready for discussion.
Can lifestyle changes affect testosterone levels?
Yes, factors like diet, exercise, stress, sleep, and weight can influence testosterone. Maintaining a healthy lifestyle—such as regular physical activity, balanced nutrition, and adequate sleep—may support stable hormone levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy