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Testosterone, Serum (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of testosterone, a key hormone involved in male and female health, including muscle development, bone density, and sexual function.

Quick summary

["Testosterone levels help assess hormone balance and potential health concerns like low testosterone or hormonal imbalances."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
100838-2
Short code
Testost sp9 p chal SerPl-mCnc
Synonyms
Testost sp9 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your doctor may order a testosterone test to check for conditions like low testosterone (hypogonadism), high testosterone (hyperandrogenism), or to monitor hormonal treatments. It can also help diagnose symptoms such as fatigue, changes in libido, or irregular menstrual cycles.

What Does the Test Involve?

A blood sample is taken from a vein in your arm. The sample is sent to a lab where it is processed to measure testosterone levels. No special preparation is usually needed, but fasting may be required depending on the lab’s protocol.

What Are Common Reasons for Testing?

Testosterone levels may be checked if you have symptoms like low energy, reduced muscle mass, or changes in mood. It’s also used in fertility evaluations, monitoring hormone replacement therapy, or assessing conditions like polycystic ovary syndrome (PCOS) in women.

How Is the Test Performed?

The blood sample is collected in a sterile tube and sent to a lab for analysis. Testosterone is measured using a highly sensitive immunoassay method, which detects the hormone’s presence and concentration in your blood.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Serum Testosterone

Frequently asked questions

Your testosterone levels can indicate whether you have normal hormone function or if there’s an underlying issue affecting your health. Low testosterone may lead to fatigue, reduced sex drive, or muscle weakness, while high levels could cause acne, irregular periods, or other hormonal symptoms. Always discuss results with your healthcare provider.
How long does it take to get results from this test?
Testosterone results are typically available within 1–3 business days, depending on the lab and your healthcare provider’s workflow. Your doctor will contact you once the results are ready.
What if my testosterone levels are outside of the expected range?
Does fasting affect testosterone test results?
Sometimes fasting is required before testing to ensure accurate results, but this depends on the lab’s specific instructions. Your doctor or lab technician will provide guidance if needed.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy