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Total Serum Testosterone

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. Testosterone helps regulate muscle and bone growth, as well as sex drive and sperm production.

Quick summary

["Total testosterone levels can help diagnose hormonal imbalances or conditions like low testosterone or fertility issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
2987-6
Short code
Testost Ur Ql
Synonyms
Testost Ur Ql
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your doctor may recommend this test if you have symptoms like low libido, fatigue, reduced muscle mass, or difficulty with fertility. It could also be part of a broader evaluation for conditions like hypogonadism (low testosterone) or Cushing’s syndrome (high testosterone).

What to Expect Before the Test

No special preparation is needed, but if you’re taking medications that affect hormones (like birth control or steroids), let your doctor know. Avoid alcohol for 24 hours before testing, as it may affect hormone levels.

How the Test Works

A blood sample is taken from a vein in your arm. The sample is sent to a lab where it is analyzed to measure total testosterone levels using a sensitive immunoassay method.

What the Results Mean

Results are compared to reference ranges for your age and sex, but individual results can vary. High or low levels may signal underlying health concerns that require further evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Testosterone

Frequently asked questions

How often should I get this test?
The frequency depends on your symptoms and medical history. Your doctor may recommend testing every few months or annually if you have ongoing concerns about hormone levels.
What causes low or high testosterone levels?
Low testosterone can result from conditions like pituitary disorders, kidney disease, or aging. High testosterone may be linked to conditions like Cushing’s syndrome, tumors, or genetic factors. Lifestyle factors like obesity, smoking, or poor sleep can also influence hormone levels.
Can diet or exercise affect testosterone levels?
Yes! A balanced diet rich in zinc, vitamin D, and healthy fats, along with regular exercise, may support healthy testosterone levels. Stress, poor sleep, and excessive alcohol intake can lower them, so lifestyle habits play a role.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy