Tetramethylbenzidine (TMB) substrate concentration in a chemiluminescent immunoassay (CLIA) probe for detecting antibody-antigen interactions (e.g., in certain infectious disease or autoimmune testing)

What This Test Measures

The test uses a chemical process called chemiluminescence, where a substrate (TMB) reacts with antibodies or antigens bound to a probe. This reaction produces a measurable light signal, which is recorded to determine the presence or level of the target substance in your blood.

How the Test Works

Your blood sample is processed in a specialized machine that detects the chemical reaction. The machine converts the signal into a result, which may be reported qualitatively (positive/negative) or as a measured concentration depending on the clinical context.

Common Uses

This type of assay is commonly used in screening or confirmation tests for conditions such as infections (e.g., certain viruses), autoimmune diseases, or other immune-related disorders. It may also be used in research or specialized diagnostic settings.

Sample Collection and Preparation

A blood sample is typically collected from a vein in your arm. The sample is then processed and sent to a lab where it undergoes the chemiluminescent immunoassay procedure. The test may be performed on serum or plasma, depending on the specific clinical protocol.