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Folate concentration in cerebrospinal fluid (CSF)

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of folate, a B vitamin, in your spinal fluid. Folate helps your body make healthy red blood cells and DNA.

Quick summary

["Folate in CSF may help diagnose or monitor conditions affecting your nervous system."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
75048-9
Short code
THfolate CSF-sCnc
Synonyms
THfolate CSF-sCnc
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Ref low
2.7
Ref high
17
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

Why is this test done?

Your doctor may order this test if you have symptoms like numbness, weakness, or trouble walking that could be linked to folate deficiency or certain neurological disorders. It’s also used to check for conditions like multiple sclerosis or other spinal cord issues where folate levels might be important.

What does the test involve?

A small amount of fluid is taken from your lower back through a procedure called a lumbar puncture (spinal tap). The fluid is then sent to the lab for testing.

What should you expect before the test?

You may need to stay still for a few hours before the test to help with fluid balance. Wear comfortable clothing with an open back, and bring any medications you’re taking with you.

What happens during the test?

A doctor or nurse will insert a needle into your lower back and carefully remove a small sample of CSF. You might feel pressure or a slight pinch, but it’s usually over quickly. You’ll lie flat afterward to help prevent any headaches.

Reference range

NCBI: Folate 2.7-17.0 ng/mLN/A

Specimen: csf

Fasting for 8-12 hours preferred for some tests. Inform about supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Folate CSF

Frequently asked questions

What does a low or high folate level in CSF mean?
A low folate level in CSF might suggest a folate deficiency or conditions like multiple sclerosis, where folate metabolism is disrupted. High levels could indicate certain infections, inflammation, or other neurological conditions that require medical attention.
How long does it take to get results?
Results are typically available within 1 to 3 business days, depending on your lab’s processing time. Your doctor will discuss the results with you during your follow-up visit.
Can I eat or drink before the test?
You can usually eat and drink normally before the test unless your doctor advises otherwise. Avoid caffeine or alcohol for a few hours before the procedure to help reduce discomfort.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy