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Threonine, Cerebrospinal Fluid, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of threonine, an amino acid, in cerebrospinal fluid (the fluid surrounding the brain and spinal cord).

Quick summary

["Threonine measurement in CSF can help evaluate certain metabolic or nutritional conditions affecting the central nervous system."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
3006-4
Short code
Threonine CSF-mCnc
Synonyms
Threonine CSF-mCnc
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

What This Test Measures

Threonine is one of the building blocks of protein (an amino acid) that the body uses to make proteins and other important molecules. This test determines how much threonine is present in a sample of cerebrospinal fluid, which is collected through a procedure called a lumbar puncture (spinal tap).

Why Doctors Order It

Your healthcare provider may order this test when evaluating suspected amino acid metabolism disorders, unusual neurological symptoms, or nutritional concerns. It is often part of a broader panel of amino acid testing rather than a stand-alone test, and results are interpreted alongside other findings.

How the Sample Is Collected

Cerebrospinal fluid is collected by a clinician during a lumbar puncture, where a thin needle is placed in the lower back to draw a small sample of fluid. The sample is then sent to a laboratory for analysis. Your care team will explain any preparation steps beforehand.

What Results Mean

Results are reported as a mass concentration of threonine in the fluid. Whether a value is considered normal depends on the laboratory's own reference range and on the individual's age and clinical context. Your provider will interpret the result in light of your symptoms and other test results, so discuss any questions directly with them.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Threonine levels in cerebrospinal fluid can be relevant in the workup of inherited amino acid metabolism disorders and some neurological or nutritional conditions. Because cerebrospinal fluid testing is specialized and reference ranges vary by laboratory, results should always be interpreted by a qualified clinician in the context of the full clinical picture.

Short name

CSF Threonine

Frequently asked questions

Is this test the same as a blood amino acid test?
No. This test specifically measures threonine in cerebrospinal fluid, which is different from measuring amino acids in blood or urine. Each specimen type provides different information, and your provider will choose the appropriate test.
Does an abnormal result mean I have a disease?
Not necessarily. Results outside a reference range can occur for many reasons, and interpretation depends on your age, symptoms, and other tests. Your healthcare provider is the best person to explain what your result means for you.
Is a lumbar puncture painful?
Most people feel a brief sting or pressure when the numbing medication is given and during needle placement, but the procedure is generally well tolerated. Your care team will discuss the process, risks, and aftercare with you beforehand.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy