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Anti-Blood Group Antibody Screening (Qualitative) by Line Immunoblot

Published: August 9, 2026Updated: August 16, 2026

This test checks for antibodies in your blood that may react against red blood cells, often done before certain blood transfusions or pregnancies to prevent complications.

Quick summary

["Helps ensure safe blood transfusions or pregnancy outcomes by detecting unexpected antibodies that could cause problems."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
81743-7
Short code
Th-To Ab Ser Ql Line blot
Synonyms
Th-To Ab Ser Ql Line blot
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
line-blot
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you’re preparing for a blood transfusion, expecting a baby, or experiencing symptoms like anemia, jaundice, or unusual bleeding that might suggest an immune reaction to red blood cells.

What to Expect During the Test

A small blood sample is taken from your arm. The sample is then tested using a line immunoblot method to detect specific antibodies against blood group antigens.

How the Results Are Interpreted

Results are qualitative (positive or negative) and indicate whether antibodies are present. A positive result means antibodies were found, which may require further testing or special precautions.

Common Reasons for Testing

This screening is critical for patients at risk of hemolytic disease of the fetus and newborn (HDFN), those needing blood transfusions, or those with autoimmune conditions affecting red blood cells.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Anti-Rh/ABO Antibody Screen (Qualitative)

Frequently asked questions

What does a positive result mean?
A positive result means antibodies were detected in your blood. This may need further testing (like confirmation via another method) or additional monitoring, depending on your medical situation.
Will I need to prepare for this test?
No special preparation is needed. You can eat, drink, or take medications as usual on the day of the test.
How long will it take to get my results?
Results are typically available within 24 to 48 hours, depending on lab processing times.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy