Skip to content

Anti-double-stranded DNA antibody (dsDNA) test

Published: August 9, 2026Updated: August 16, 2026

This test measures the presence of antibodies against double-stranded DNA, a component found in the nucleus of cells. These antibodies can be linked to certain autoimmune conditions.

Quick summary

["This test helps diagnose or monitor autoimmune diseases affecting your immune system."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
43238-5
Short code
T palldm Ab Ser Donr Ql IF
Synonyms
T palldm Ab Ser Donr Ql IF
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
if
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms or signs of an autoimmune disease, such as unexplained joint pain, skin rashes, or fatigue. It may also be used to monitor conditions like lupus or other connective tissue disorders.

What to Expect Before the Test

No special preparation is usually needed. You may be asked to fast before the blood draw, but this is not always required. Bring any medications you’re currently taking to your appointment.

How the Test Works

A small amount of blood is drawn from a vein in your arm. The blood sample is then tested in a lab to detect the presence of antibodies against double-stranded DNA.

Possible Results and Their Meaning

Results can be positive (indicating the presence of these antibodies) or negative. A positive result may suggest an autoimmune condition, but it doesn’t confirm a diagnosis alone. Your healthcare provider will review the results in the context of your symptoms and other tests.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated levels of anti-dsDNA antibodies are strongly associated with systemic lupus erythematosus (SLE) and other autoimmune diseases. They can help guide treatment decisions and monitor disease activity, but further evaluation with other tests and clinical findings is necessary for a definitive diagnosis.

Short name

dsDNA Antibody

Frequently asked questions

What does a positive result mean?
A positive result suggests an increased likelihood of an autoimmune condition, such as lupus, but it doesn’t confirm the diagnosis by itself. Your doctor will consider your symptoms, medical history, and other test results to make an accurate assessment.
Can this test be used to diagnose lupus?
While this test is highly sensitive for certain autoimmune conditions, including lupus, it is not definitive on its own. Doctors often use it alongside other tests and clinical findings to diagnose or monitor these conditions.
How often will I need this test?
Your healthcare provider will determine how often you need this test based on your symptoms, diagnosis, and treatment plan. Some patients may need it periodically to monitor disease activity or response to treatment.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy