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Total cotinine in saliva (mass concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cotinine, a metabolite of nicotine, found in your saliva. Cotinine helps track recent exposure to tobacco smoke or nicotine products.

Quick summary

["Cotinine in saliva helps assess recent nicotine exposure."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
78887-7
Short code
trans-3-OH-cotinine Sal-mCnc
Synonyms
trans-3-OH-cotinine Sal-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why is this test done?

Your healthcare provider may order this test to evaluate exposure to tobacco smoke or nicotine products, such as cigarettes, vaping, or chewing tobacco. It can also be used in research or clinical studies to monitor nicotine use or withdrawal.

What does a result mean?

Higher levels of cotinine in saliva typically indicate recent exposure to nicotine. However, interpretation depends on clinical context, such as the timing of the test relative to exposure or the presence of other factors like diet or medications.

How is the test performed?

A small saliva sample is collected, usually by spitting into a provided container. The sample is then analyzed in a lab using a sensitive detection method to measure cotinine levels.

What should I expect before the test?

No special preparation is needed. You may eat or drink normally, but avoid chewing tobacco or smoking immediately before testing to ensure accurate results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Cotinine in saliva

Frequently asked questions

How soon after exposure can I expect accurate results?
Cotinine levels in saliva peak within minutes to hours after nicotine exposure and can remain detectable for several hours. However, results should be interpreted by your healthcare provider based on clinical context, such as the timing of the test relative to exposure.
Can food or medications affect cotinine levels?
Some foods or medications may influence nicotine metabolism, but saliva cotinine levels are generally less affected by diet than urine or blood tests. Always follow your provider’s instructions regarding timing and preparation.
Is this test painful or uncomfortable?
No, this test is completely painless. You simply provide a saliva sample, similar to spitting into a cup, and it does not require needles or blood draws.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy