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Cortisol in Cerebrospinal Fluid (CSF)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of cortisol, a stress hormone, in your cerebrospinal fluid (the fluid surrounding your brain and spinal cord). It helps evaluate conditions affecting the brain’s production or response to cortisol.

Quick summary

["Assessing cortisol levels in CSF can help diagnose neurological or endocrine disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
18378-0
Short code
Transcortin CSF-mCnc
Synonyms
Transcortin CSF-mCnc
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like severe headaches, seizures, or neurological issues that could be linked to abnormal hormone levels in the brain. It’s also used in research or specialized cases where cortisol’s role in CSF is suspected, such as in pituitary or adrenal disorders.

What to Expect Before the Test

No special preparation is needed. You’ll have a small needle inserted into your lumbar spine to collect a sample of CSF. This is typically done in a hospital or clinic setting under sterile conditions.

What the Test Results Mean

Cortisol in CSF is usually measured to compare it with normal levels. High or low results may indicate conditions like Cushing’s syndrome (excess cortisol), adrenal insufficiency, or other brain-related disorders. Your doctor will interpret results in the context of your symptoms and medical history.

Possible Causes of Abnormal Results

Elevated cortisol in CSF might suggest conditions like brain tumors, meningitis, or certain neurological infections. Low levels could be linked to pituitary or adrenal gland dysfunction. Always discuss findings with your healthcare provider.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CSF Cortisol

Frequently asked questions

What triggers this test?
This test is usually ordered if you have unexplained neurological symptoms, such as severe headaches, vision changes, or seizures, that might involve abnormal hormone levels in the brain. It may also be part of a research study or specialized diagnostic workup.
How is the CSF sample collected?
A healthcare provider will insert a needle into your lower back to collect a small amount of CSF. This procedure is called a lumbar puncture (spinal tap) and is done in a sterile environment. You may feel pressure or a slight ache afterward.
How long does it take to get results?
Results are typically available within a few days to a week, depending on lab processing times. Your doctor will contact you once they are ready for review.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy