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Total Cholesterol (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of cholesterol in your blood, which includes both low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol, as well as triglycerides.

Quick summary

["Cholesterol levels help assess your heart health risk."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
14445-1
Short code
Trigl Amn-mCnc
Synonyms
Trigl Amn-mCnc
Category
glucose
Sample
fluid
Container
Sterile tube
Units
N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
2-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
Hemolysis max
300
TAT
4–24 hours
Order
routine

Why This Test Matters

Cholesterol is a type of fat that your body needs for normal cell function, but having too much can increase your risk of heart disease and stroke. High cholesterol levels often don’t cause symptoms, so regular testing is important to stay informed about your health.

What to Expect During the Test

A blood sample is taken from a vein, usually in your arm, by a healthcare professional. You may feel a slight pinch, but it typically doesn’t cause discomfort afterward. The sample is sent to a lab for analysis.

Factors That Can Affect Results

Several factors can influence cholesterol levels, including diet (especially foods high in saturated fats), physical activity, genetics, and certain medications. Stress, weight, and smoking can also play a role. If you’re taking medications, let your provider know before testing.

Preparation for the Test

For most cholesterol tests, you may need to fast for 9–12 hours before drawing blood to get the most accurate results. Avoid eating or drinking anything except water during this time. If you have diabetes, check with your provider first, as fasting rules may differ.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Cholesterol

Frequently asked questions

What does a high or low cholesterol level mean?
High cholesterol levels (above normal ranges) are often a silent risk factor for heart disease. LDL cholesterol is particularly important because it can build up in your arteries over time, narrowing them and increasing the risk of heart attacks or strokes. HDL cholesterol, sometimes called 'good cholesterol,' helps remove LDL cholesterol from your arteries. Your provider will discuss your results and any necessary lifestyle or medical interventions.
What if my cholesterol is out of range?
If your cholesterol test results are outside the recommended range, your healthcare provider may recommend changes to your diet, exercise routine, or medications to help manage your levels. They’ll work with you to create a plan tailored to your needs.
How often should I get this test?
Healthcare providers typically recommend cholesterol screening every 4–5 years for adults aged 20 and older, or more frequently if you have risk factors like high blood pressure, diabetes, or a family history of heart disease. Regular monitoring helps you stay proactive about your heart health.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy