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Thyroid Stimulating Hormone (TSH) with Confirmatory Immunoassay (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures your thyroid-stimulating hormone levels in your blood to help diagnose or monitor thyroid conditions, such as hypothyroidism or hyperthyroidism.

Quick summary

["A confirmatory TSH test helps ensure accurate results for thyroid function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
56594-5
Short code
TSH 3h p chal SerPl-aCnc
Synonyms
TSH 3h p chal SerPl-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.4
Ref high
4
Critical range
0.1 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84443
SNOMED
301343003
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like fatigue, weight changes, or unexplained goiter (enlarged thyroid). It may also be used to monitor thyroid hormone treatment or screen for thyroid disorders in certain groups, such as pregnant women or those with family history of thyroid disease.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if your blood is drawn early in the morning. Avoid smoking or caffeine for at least 30 minutes before your visit.

During the Test

A healthcare professional will draw blood from a vein in your arm. You may feel a slight pinch, but the process usually takes just a few minutes. The blood sample is then sent to a lab for analysis using an immunoassay method to detect TSH levels.

What the Results Mean

Results are interpreted by your healthcare provider based on your symptoms, medical history, and other tests. Abnormal results may prompt further evaluation, such as additional thyroid function tests or imaging.

Reference range

Adult: 0.4-4.0 mIU/LN/A

Specimen: serum

Take your thyroid medication after the blood draw if applicable. No fasting required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Confirmatory TSH

Frequently asked questions

What does a high TSH level usually indicate?
A high TSH level often suggests hypothyroidism (underactive thyroid), where your thyroid isn’t producing enough hormones. It may also occur in conditions like pituitary disorders or certain medications.
What does a low TSH level usually indicate?
A low TSH level may signal hyperthyroidism (overactive thyroid), where your thyroid produces too many hormones. It can also occur in thyroiditis (temporary inflammation) or thyroid cancer in some cases.
Why might my TSH test be repeated?
Your provider may repeat the test if initial results are unclear, if you’re on thyroid hormone medication, or if you have symptoms that don’t align with the test results. Follow-up may include additional tests or adjustments to treatment.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy