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Thyroid Stimulating Hormone (TSH) with Immunoassay Confirmation

Published: August 9, 2026Updated: August 16, 2026

Your blood test measures thyroid-stimulating hormone (TSH), a key hormone that helps regulate your thyroid function. This test confirms the results of an initial screening.

Quick summary

["This test helps assess whether your thyroid is working normally or if it may be underactive or overactive."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40673-6
Short code
TSH BII Ser-aCnc
Synonyms
TSH BII Ser-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.4
Ref high
4
Critical range
0.1 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84443
SNOMED
301343003
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you have symptoms like fatigue, weight changes, or unexplained mood swings. It’s also used to monitor thyroid conditions, such as hypothyroidism or hyperthyroidism, or to check for thyroid dysfunction before or after treatment.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if it’s part of a fasting panel. Bring any medications you’re currently taking to your appointment.

How the Test Works

A small blood sample is drawn from a vein in your arm. The blood is sent to a lab where it’s tested for TSH levels using an immunoassay method, which detects the hormone in your blood.

What the Results Mean

High or low TSH levels can indicate thyroid issues, but your doctor will interpret the results in relation to your symptoms and medical history. Results are not given as a standalone number but as a comparison to expected ranges.

Reference range

Adult: 0.4-4.0 mIU/LN/A

Specimen: serum

Take your thyroid medication after the blood draw if applicable. No fasting required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

TSH levels are critical for diagnosing and managing thyroid disorders. Low TSH may suggest hyperthyroidism (overactive thyroid), while high TSH often points to hypothyroidism (underactive thyroid). Confirmatory testing ensures accurate diagnosis and guides treatment decisions, such as medication adjustments or further testing.

Short name

TSH Confirmation

Frequently asked questions

What does a high TSH level mean?
A high TSH level may indicate your thyroid isn’t producing enough hormone, which could be due to conditions like Hashimoto’s disease or hypothyroidism. Your doctor will discuss next steps based on your symptoms and test results.
What does a low TSH level mean?
A low TSH level may suggest your thyroid is overactive, possibly from conditions like Graves’ disease or a thyroid tumor. This result often prompts further evaluation, such as additional thyroid tests or imaging.
How often should I get this test?
Your doctor will determine how often you need this test based on your health status, symptoms, and treatment plan. Regular monitoring may be needed if you have a thyroid condition or take thyroid medication.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy