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Thyroid Stimulating Hormone (TSH) with Free Thyroxine (FT4) and Free Triiodothyronine (FT3) Profile

Published: August 9, 2026Updated: August 16, 2026

This test measures key hormones involved in regulating your thyroid function, including thyroid-stimulating hormone (TSH), free thyroxine (FT4), and free triiodothyronine (FT3). It helps assess whether your thyroid is working normally or if there may be an overactive or underactive condition.

Quick summary

["This panel helps diagnose and monitor thyroid disorders by checking hormone balance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
69561-9
Short code
TSH BldCo-aCnc
Synonyms
TSH BldCo-aCnc
Category
chemistry
Sample
whole-blood
Container
Lavender-top (EDTA)
Units
N/A
Ref low
0.4
Ref high
4
Critical range
0.1 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84443
SNOMED
301343003
Specimen volume
3 mL
Stability
Room temperature: 4h, Refrigerated: 24h
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Matters for Your Health

Your thyroid gland produces hormones that control metabolism, energy levels, and many other bodily functions. If your thyroid isn’t working properly, it can lead to fatigue, weight changes, or other symptoms. This test helps doctors identify whether your thyroid is too active or too slow, guiding treatment decisions.

What Hormones Are Being Checked?

TSH (Thyroid-Stimulating Hormone) is produced by your pituitary gland and signals the thyroid to make more hormones. FT4 (Free Thyroxine) and FT3 (Free Triiodothyronine) are the active forms of thyroid hormones circulating in your blood. These three together give a full picture of your thyroid health.

When Might You Need This Test?

Doctors may order this test if you have symptoms like unexplained weight changes, fatigue, hair loss, or mood swings. It’s also used to monitor thyroid conditions like hypothyroidism or hyperthyroidism, especially after treatment for thyroid disorders.

What to Expect Before the Test

No special preparation is needed for this test. You’ll have a small blood sample drawn from a vein, usually in your arm. It usually takes about 15 minutes, and you can resume normal activities afterward. Avoid smoking or drinking alcohol for a few hours before the test if instructed.

Reference range

Adult: 0.4-4.0 mIU/LN/A

Specimen: whole-blood

Take your thyroid medication after the blood draw if applicable. No fasting required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Thyroid Function Panel

Frequently asked questions

What does a ‘normal’ result mean for me?
A ‘normal’ result doesn’t mean your thyroid is perfectly healthy—it just means your hormone levels fall within the typical range for most people. Your doctor will consider your symptoms, medical history, and other test results to determine if further action is needed.
Can I eat or drink anything before this test?
You can eat and drink normally before this test unless your doctor specifies otherwise. Some tests may require fasting, but this one does not. Always follow your healthcare provider’s instructions.
Will this test affect my medication?
If you’re taking thyroid hormone medication, let your doctor know before the test. They may adjust your dose based on the results. Some medications can interfere with test accuracy, so it’s important to communicate all current treatments.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy