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Thyroid-Stimulating Hormone (TSH) with Immunoassay Method

Published: August 9, 2026Updated: August 16, 2026

This blood test measures your thyroid-stimulating hormone level, which helps your body regulate metabolism and thyroid hormone production.

Quick summary

["TSH helps doctors check if your thyroid is working properly."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
29575-8
Short code
TSH DBS-aCnc
Synonyms
TSH DBS-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.4
Ref high
4
Critical range
0.1 N/A – 20 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84443
SNOMED
301343003
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order a TSH test to diagnose or monitor thyroid conditions, such as hypothyroidism (underactive thyroid) or hyperthyroidism (overactive thyroid). It’s also used during pregnancy, to check for thyroid issues before surgery, or to evaluate symptoms like fatigue, weight changes, or unexplained goiter.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the blood draw if your test is part of a more complex evaluation. Wear comfortable clothing and plan for the test to take about 15 minutes. Avoid smoking or caffeine before the draw if instructed.

What Happens During the Test

A healthcare provider will insert a small needle into a vein in your arm to draw blood. You may feel a slight pinch, but it usually doesn’t cause discomfort. The blood sample is sent to a lab for analysis using an immunoassay method to measure TSH levels.

What the Results Mean

Results are interpreted based on your symptoms, medical history, and other tests. Low TSH levels may suggest hyperthyroidism, while high levels may indicate hypothyroidism. Your doctor will discuss findings with you and may recommend follow-up tests or treatments.

Reference range

Adult: 0.4-4.0 mIU/LN/A

Specimen: serum

Take your thyroid medication after the blood draw if applicable. No fasting required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

TSH is a critical marker for thyroid function. Abnormal levels can signal thyroid disorders that may affect energy levels, digestion, heart function, and mood. Early detection helps manage conditions like Hashimoto’s disease or Graves’ disease, which can improve long-term health outcomes.

Short name

TSH

Frequently asked questions

What causes TSH levels to be too high or too low?
High TSH levels often result from hypothyroidism (e.g., due to Hashimoto’s thyroiditis, pituitary issues, or iodine deficiency). Low TSH levels may signal hyperthyroidism (e.g., from Graves’ disease, thyroid nodules, or excess thyroid hormone). Other factors, like pregnancy, certain medications, or thyroid surgery, can also influence results.
Can diet or lifestyle affect TSH test results?
Dietary changes (e.g., high iodine intake from supplements or seafood) or lifestyle factors (e.g., stress, sleep patterns) can temporarily impact thyroid function. However, TSH results are generally reliable unless your doctor specifies fasting or other restrictions. Always follow their instructions.
How often should I get a TSH test?
Your doctor will determine the frequency based on your health status. Regular monitoring may be needed if you have a thyroid condition, take thyroid medication, or are at higher risk for thyroid disorders. Follow-up tests could be scheduled every 6 months to a year, depending on your needs.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy