24-Hour Urine Test for Estradiol (Substance Concentration)
This test measures the total amount of estradiol, a key female sex hormone, collected over a full day in your urine. It helps assess hormone balance, especially in conditions affecting estrogen levels.
["This test helps evaluate estrogen production and potential hormonal imbalances over a 24-hour period."]
Key facts at a glance
- LOINC
- 34288-1
- Short code
- u Estradiol 24h Ur-sCnc
- Synonyms
- u Estradiol 24h Ur-sCnc
- Category
- chemistry
- Sample
- urine
- Container
- Sterile urine container
- Units
- N/A
- Ref low
- 20
- Ref high
- 400
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 82670
- SNOMED
- 300919004
- Specimen volume
- 10 mL
- Stability
- Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Doctors may order a 24-hour urine estradiol test to check for hormonal issues, such as low or high estrogen levels, which can affect fertility, menstrual cycles, or other reproductive health concerns. It’s also used in monitoring conditions like ovarian dysfunction, hormone therapy, or certain cancers linked to estrogen.
How the Test Works
You’ll collect all your urine over a 24-hour period (typically from one morning to the next morning) in a special container. The collected urine is then sent to a lab for analysis to determine the total amount of estradiol present.
Sample Collection Tips
To ensure accurate results, follow your doctor’s instructions carefully. Drink plenty of water to help flush out hormones naturally, but avoid certain medications or foods that might interfere with the test. Missing any urine or collecting it incorrectly can affect the outcome.
What to Expect Before the Test
No special preparation is usually needed, but avoid smoking or drinking alcohol before the test as they might influence hormone levels. If you’re on hormone therapy, discuss it with your doctor to ensure it doesn’t interfere with the results.
Reference range
Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A
For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Elevated estradiol levels may suggest conditions like ovarian tumors, early puberty in adolescents, or certain cancers. Low levels could indicate menopause, ovarian failure, or hormonal disorders. This test helps guide treatment decisions for reproductive health, fertility, or endocrine disorders.
Short name
24-Hour Urine Estradiol
Frequently asked questions
How long does the 24-hour urine collection take?
Can I drink alcohol or smoke during the urine collection?
Will I need to fast before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy