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24-Hour Urine Test for Estradiol (Total)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of estradiol, a form of estrogen, collected in your urine over a 24-hour period. It helps assess estrogen levels in your body.

Quick summary

["Understanding your estrogen levels over time can help diagnose hormonal imbalances or track treatment effectiveness."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
15062-3
Short code
u Estradiol 24h Ur-sRate
Synonyms
u Estradiol 24h Ur-sRate
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms or conditions linked to estrogen, such as irregular menstrual cycles, symptoms of perimenopause, or suspected hormonal disorders. It can also be used to monitor treatment for conditions like breast cancer or endometriosis.

What to Expect Before the Test

You’ll need to collect all urine for a full 24-hour period, typically starting after you urinate in the morning. Store the urine in a refrigerator until you return it to the lab. Follow your provider’s instructions precisely to ensure accurate results.

What Happens During the Test

No special preparation is needed beyond collecting urine over 24 hours. The lab will analyze the urine sample to measure the total estradiol concentration.

What the Results Mean

Results are not provided with specific reference ranges. Your provider will interpret the results in relation to your symptoms, medical history, and other tests. High or low levels may indicate conditions like hormonal imbalances, tumors, or other health issues.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: urine

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

24h Urine Estradiol

Frequently asked questions

What does this test measure?
This test measures the total amount of estradiol, a primary estrogen hormone, excreted in your urine over 24 hours. Estradiol is crucial for reproductive health, bone density, and overall hormonal balance. It helps identify whether your estrogen levels are too high or too low, which can affect fertility, menstrual cycles, and other health conditions.
Can this test detect cancer?
Yes, elevated estradiol levels in urine can sometimes be associated with certain types of hormone-related cancers, such as breast or endometrial cancer. However, this test alone is not diagnostic for cancer. Your provider may order additional tests to confirm or rule out a diagnosis.
How often should I get this test?
The frequency of this test depends on your medical needs. Your healthcare provider will determine how often to repeat it based on your symptoms, treatment plan, or monitoring requirements. Regular follow-ups may be recommended if you have conditions like perimenopause, hormonal therapy, or suspected tumors.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy