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Estrogen (Estradiol) in Urine, 24-Hour Collection

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of estradiol, a primary female sex hormone, over a full day in your urine. It helps assess hormone balance, especially in conditions affecting estrogen levels.

Quick summary

["This test provides a comprehensive picture of your daily estradiol exposure, useful for diagnosing hormonal imbalances or tracking treatment responses."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
110449-6
Short code
u Estradiol Msmt Ur
Synonyms
u Estradiol Msmt Ur
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like irregular menstrual cycles, bone density concerns, or suspected hormonal disorders. It’s also used in research or clinical studies evaluating estrogen metabolism.

How the Test Works

You’ll collect all urine for a 24-hour period in a special container. The sample is then sent to a lab for analysis, typically using immunoassay methods to measure estradiol concentration.

Sample Collection Tips

Start and end the collection at the same time each day (e.g., midnight to midnight). Empty your bladder before starting, then collect every void. Store urine in a cool place until you return it promptly.

What to Expect Before the Test

No special preparation is needed, but avoid using estrogen-containing medications or supplements for at least 24 hours before testing. Inform your provider of any medications or supplements you’re taking.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: urine

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Estradiol

Frequently asked questions

How long does it take to get results?
Results are typically available within 1–2 weeks after the lab processes your sample. Follow-up with your provider to discuss findings.
What does a ‘normal’ range mean?
There’s no single ‘normal’ range for 24-hour urine estradiol because levels vary widely by age, sex, and health status. Your provider will compare your results to your baseline or clinical context.
Can diet or exercise affect this test?
Dietary supplements (e.g., phytoestrogens) or certain medications (e.g., birth control) may influence estradiol levels. Exercise generally doesn’t directly impact estradiol, but intense physical activity could temporarily alter hormone metabolism.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy