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Estrogen (specifically estrone sulfate) concentration in urine

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estrone sulfate, a form of estrogen, found in your urine over a 24-hour period. It helps assess estrogen levels, often used in reproductive health evaluations.

Quick summary

["This test helps track hormone balance, especially useful for monitoring estrogen levels in conditions like menopause or fertility treatments."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
34289-9
Short code
u Estradiol Ur-sCnc
Synonyms
u Estradiol Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order a 24-hour urine estrone sulfate test to evaluate estrogen production, diagnose hormonal imbalances, or monitor treatment responses in conditions such as ovarian function, breast cancer, or menopause. It can also be part of fertility assessments or endocrine evaluations.

What to Expect Before the Test

No special preparation is needed, but you’ll collect your urine over a full 24-hour period, starting after you’ve emptied your bladder at the beginning of the day. Store the urine samples properly to preserve accuracy.

How the Test Works

The collected urine is analyzed to measure estrone sulfate levels, which are then compared to reference ranges to determine your hormone status. The method typically involves specialized laboratory techniques to detect and quantify the substance.

Common Uses and Conditions

This test is commonly used to diagnose or monitor conditions like ovarian dysfunction, breast cancer (as part of hormone receptor testing), or hormonal therapy adjustments. It may also help assess estrogen dominance or evaluate reproductive health in premenopausal women.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: urine

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Urine Estrone Sulfate

Frequently asked questions

How long does it take to get the results of this test?
Results are typically available within 1–3 business days, depending on lab processing times. Your healthcare provider will discuss the findings with you during your follow-up visit.
What do my estrone sulfate levels mean?
Estrone sulfate levels can indicate overall estrogen activity in your body. Elevated levels may suggest conditions like ovarian tumors or hormonal imbalances, while lower levels could relate to menopause or reduced ovarian function. Your doctor will interpret results in the context of your medical history and symptoms.
Can diet or medications affect this test?
Yes, certain foods, supplements, or medications—such as phytoestrogens (e.g., soy products) or hormonal therapies—may influence estrone sulfate levels. Avoid these if possible during your urine collection period to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy