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Vitamin B12 (Serum) with Immunoassay Confirmation

Published: August 9, 2026Updated: August 17, 2026

This test measures the level of vitamin B12 in your blood to check if you have enough of this essential nutrient, which is important for nerve function and red blood cell production.

Quick summary

["Vitamin B12 helps prevent nerve damage and anemia; low levels may need dietary or medical attention."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
30905-4
Short code
Vit B12 p B12+IF/pB12 24H Ur-aCnc
Synonyms
Vit B12 p B12+IF/pB12 24H Ur-aCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
200
Ref high
900
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your doctor may order this test if you have symptoms like fatigue, numbness, tingling in hands/feet, or digestive issues. It’s also used to diagnose or monitor conditions like pernicious anemia, vitamin B12 deficiency, or malabsorption disorders.

What to Expect Before the Test

No special preparation is needed, but inform your doctor about any medications (especially those affecting blood or nerves) or recent vitamin supplements. Avoid alcohol before testing if advised.

What the Test Results Mean

Results help determine if your vitamin B12 levels are within normal range. Low levels may indicate deficiency, while high levels could signal other health concerns like liver disease or certain cancers.

Common Conditions Linked to Results

Low vitamin B12 can cause anemia, nerve damage, or cognitive issues. High levels may require further evaluation by a specialist, such as a hematologist or endocrinologist.

Reference range

NCBI: Vitamin B12 200-900 pg/mLN/A

Specimen: urine

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Vitamin B12 plays a critical role in red blood cell production, nerve function, and DNA synthesis. Deficiency can lead to serious long-term health problems if untreated, including neurological damage or heart disease.

Short name

Vitamin B12 Blood Test

Frequently asked questions

What causes vitamin B12 deficiency?
Deficiency often results from poor diet (e.g., vegan/vegetarian diets), malabsorption issues (like celiac disease or atrophic gastritis), or conditions that reduce stomach acid production.
How is vitamin B12 deficiency treated?
Treatment typically involves vitamin B12 supplements (oral, injections, or nasal sprays) or dietary changes. Follow-up tests may be needed to monitor recovery.
Can vitamin B12 levels be affected by medications?
Yes, certain medications (e.g., proton pump inhibitors, metformin) may interfere with absorption. Always discuss all medications with your doctor before testing.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy