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Pyridoxal phosphate (PLP) and pyridoxine metabolites in serum/plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures levels of active forms of vitamin B6 (pyridoxal phosphate) and related metabolites in your blood, helping assess vitamin B6 status and its role in metabolism.

Quick summary

["Vitamin B6 is essential for nerve function, red blood cell production, and metabolism of amino acids and neurotransmitters."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
95266-3
Short code
Vit B6 + metabolites Pnl SerPl
Synonyms
Vit B6 + metabolites Pnl SerPl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like nerve pain, anemia, depression, or unusual fatigue, or if you have conditions such as alcoholism, pregnancy, or certain medications that affect vitamin B6 levels. It may also be used to monitor vitamin B6 status in patients with chronic illnesses or malabsorption disorders.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for a few hours before the blood draw. Wear comfortable clothing and plan to rest after the test. Avoid smoking or consuming alcohol for 24 hours before the procedure.

What Happens During the Test

A healthcare professional will draw blood from a vein in your arm. You may feel a slight pinch, but the procedure is quick. The blood sample is then sent to a lab for analysis using advanced techniques to measure vitamin B6 metabolites.

What the Results Mean

Results help determine if your body has enough vitamin B6 to function properly. Low levels might indicate deficiencies or poor absorption, while high levels could suggest certain metabolic conditions or medication use. Your provider will discuss these results with you in the context of your overall health.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Vitamin B6 Metabolites (Serum/Plasma)

Frequently asked questions

How long will I need to wait for my results?
Results typically take 1 to 3 business days, depending on your lab’s processing schedule. Your healthcare provider will contact you once they are ready to discuss your results.
What does it mean if my vitamin B6 levels are low?
Low levels may suggest vitamin B6 deficiency, which can cause symptoms like tingling in hands/feet, mood changes, or fatigue. Your provider may recommend dietary changes, supplements, or further testing to address the underlying cause.
Can I take vitamin B6 supplements without checking my levels first?
It’s best to avoid high-dose supplements without consulting your provider first. Excess vitamin B6 can sometimes cause side effects like nerve irritation or digestive issues. Your test results will guide whether supplementation is needed or appropriate.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy