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Vitamin C Intake Measurement (24-Hour)

Published: August 9, 2026Updated: August 16, 2026

This test estimates how much vitamin C (ascorbic acid) a person consumed over a 24-hour period, typically by analyzing a urine sample collected over a full day.

Quick summary

["A 24-hour vitamin C intake measurement helps gauge recent dietary vitamin C consumption by measuring what is excreted in the urine."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
81075-4
Short code
Vit C intake 24h Measured
Synonyms
Vit C intake 24h Measured
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
measured
CPT
82670
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Vitamin C, also called ascorbic acid, is a water-soluble vitamin found in fruits and vegetables. Because the body does not store much of it, excess amounts are passed in the urine. By measuring vitamin C in a complete 24-hour urine collection, this test estimates how much vitamin C you took in from food and supplements during that day.

How the Sample Is Collected

You will be asked to collect all of your urine over a full 24-hour period in a special container provided by the laboratory. The collection usually starts after you empty your bladder in the morning and continues until the same time the next day. The container is then returned to the lab for analysis.

What the Results Can Suggest

Results can help show whether your recent vitamin C intake is low, adequate, or high. They reflect intake over the collection day rather than your long-term vitamin C status. Your care team will interpret the result along with your diet, supplements, and other tests.

How to Prepare

Follow the laboratory's instructions about timing and storage of the collection container. Tell your care team about any vitamin C supplements you take, since they can affect the result. Do not change your diet or supplements unless your healthcare provider asks you to.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Does this test tell me if I am vitamin C deficient?
Not on its own. It estimates vitamin C intake over a 24-hour period. A deficiency is diagnosed by your healthcare provider using your symptoms, diet history, physical exam, and possibly other blood or urine tests.
Do I need to fast for this test?
Fasting is generally not required for a 24-hour urine intake measurement unless your laboratory instructs otherwise. Follow your provider's and the lab's specific preparation instructions.
Can vitamin C supplements change my result?
Yes. Taking vitamin C supplements around the collection period can raise the measured amount. Tell your care team about all supplements so the result can be interpreted correctly.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy