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Serum/Plasma Total Protein with Electrophoresis Separation (Qualitative)

Published: August 9, 2026Updated: August 16, 2026

This test measures the overall protein content in your blood and separates proteins into different categories (e.g., albumin, globulins) using electrophoresis. It helps identify abnormal protein distributions that may signal conditions like liver disease, kidney problems, or certain cancers.

Quick summary

["Assesses protein balance and potential underlying health issues through protein fraction breakdown."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
30198-6
Short code
VLCFA C26:0/C22:0 SerPl-sRto
Synonyms
VLCFA C26:0/C22:0 SerPl-sRto
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is Protein Electrophoresis Used For?

Protein electrophoresis helps doctors diagnose and monitor conditions by showing how proteins are distributed in your blood. For example, it can detect abnormal protein spikes that might indicate diseases like multiple myeloma, liver disease, or kidney disorders. It also supports evaluating immune system function by analyzing globulin levels.

How Is This Test Performed?

A small blood sample is drawn, and the serum or plasma is placed in a gel. An electric current runs through the gel, causing proteins to move based on their size and charge. Afterward, a dye stains the proteins, revealing bands that represent different protein types.

Preparation for the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if instructed by your healthcare provider. Avoid heavy meals or certain medications that could affect protein levels.

Common Conditions Monitored

This test is often used to screen for conditions like multiple myeloma (cancer of plasma cells), liver disease (e.g., cirrhosis), kidney disease, and certain autoimmune disorders. It may also help assess protein malnutrition or track treatment responses.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Protein Electrophoresis (Serum/Plasma)

Frequently asked questions

Why might my doctor order this test?
Your doctor may recommend this test if you have symptoms like unexplained weight loss, fatigue, swelling, or if they suspect conditions like cancer, liver/kidney disease, or immune disorders. It’s also used in routine follow-ups for certain chronic illnesses.
How long does it take to get results?
Results are typically available within 24–48 hours, depending on your lab’s processing time. Your healthcare provider will discuss them with you during your follow-up visit.
Can I eat or drink before this test?
Follow your doctor’s instructions, but generally, avoid heavy meals for 8–12 hours before the test. Light snacks or water are usually fine unless specified otherwise.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy