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Serum or plasma concentration of vitamin D (25-hydroxyvitamin D)

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of vitamin D in your blood, which is important for bone health and other bodily functions.

Quick summary

["Vitamin D levels help ensure your body gets enough of this essential nutrient."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
104459-3
Short code
VZV IgG CSF Ql IA
Synonyms
VZV IgG CSF Ql IA
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
CPT
82784
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your doctor may order this test to check if you have enough vitamin D, which is crucial for maintaining strong bones and preventing conditions like osteoporosis. It can also help diagnose or monitor vitamin D deficiency, which may cause symptoms like fatigue, muscle weakness, or bone pain. Sometimes, it’s used to evaluate conditions like rickets in children or osteomalacia in adults.

What Does the Test Involve?

A blood sample is taken from a vein in your arm. The blood is sent to a lab where it is processed to measure the concentration of 25-hydroxyvitamin D, a form of vitamin D that reflects your overall vitamin D status.

What Are Common Results?

Results are typically reported in nanograms per milliliter (ng/mL) or picomoles per liter (pmol/L). While exact reference ranges can vary by lab, low levels may indicate deficiency, while higher levels may suggest excess intake or certain medical conditions.

What Are Possible Causes of Abnormal Results?

Low levels of vitamin D can result from insufficient sun exposure, poor diet, limited absorption in the gut, or certain medical conditions. High levels may be due to over-supplementation or rare genetic disorders.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

25-Hydroxy Vitamin D

Frequently asked questions

Why might my vitamin D test be done even if I don’t have symptoms?
Your doctor may recommend this test if you have risk factors for deficiency, such as limited sun exposure, certain medical conditions, or taking medications that affect vitamin D absorption. Some groups, like older adults or those with darker skin, are more likely to need monitoring.
What should I do if my vitamin D test results are low?
If your results are low, your doctor may recommend increasing sun exposure, dietary changes (like eating fatty fish or fortified foods), or vitamin D supplements. They will guide you on the best approach based on your specific needs.
How often should I get this test?
The frequency depends on your health status. Generally, adults may need testing every 1–2 years if they have risk factors, while others may only need it once. Your doctor will advise based on your individual circumstances.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy