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Serum or plasma concentration of vitamin D (25-hydroxyvitamin D)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of vitamin D in your blood, which helps your body absorb calcium and maintain bone health.

Quick summary

["Vitamin D levels help assess your risk for bone-related conditions and overall health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
5403-1
Short code
VZV IgG Ser IA-aCnc
Synonyms
VZV IgG Ser IA-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters for Your Health

Vitamin D is essential for bone health, immune function, and muscle strength. Low levels may increase your risk of osteoporosis, muscle weakness, or certain infections. Your doctor may recommend this test if you have symptoms like bone pain, frequent infections, or if you’re at higher risk due to limited sun exposure, certain diets, or medical conditions.

What to Expect Before the Test

No special preparation is needed. You may have blood drawn from a vein in your arm. If you’re taking vitamin D supplements, your doctor may advise stopping them for a few days before testing to get an accurate result.

What the Results Mean

Results are typically reported as a numeric value indicating the concentration of vitamin D in your blood. Your doctor will interpret these results in relation to your overall health and symptoms.

Common Reasons for Testing

Doctors may order this test if you have symptoms like fatigue, muscle pain, or bone pain; if you have conditions like osteoporosis or rickets; or if you have limited sun exposure, certain diets (e.g., vegan), or medical conditions affecting vitamin D absorption.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Vitamin D deficiency is common and can lead to serious health issues, including weakened bones, increased infection risk, and even certain chronic diseases. Regular monitoring helps manage conditions like osteoporosis, muscle weakness, or autoimmune disorders. Your doctor will use these results to guide treatment, such as supplements or lifestyle changes.

Short name

Vitamin D Level

Frequently asked questions

What causes vitamin D deficiency?
Vitamin D deficiency can result from limited sun exposure, certain diets low in vitamin D, conditions affecting digestion (e.g., celiac disease), kidney or liver problems, or taking certain medications that interfere with vitamin D absorption.
How long does it take to get results?
Results are usually available within 1–3 business days, depending on your lab and insurance. Your doctor will contact you once they have the results.
Can I take vitamin D supplements without checking my levels first?
It’s best to check your vitamin D levels first, as taking supplements without guidance can lead to excess levels, which may cause side effects like nausea or kidney stones. Always follow your doctor’s advice.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy