Serum Protein Electrophoresis (Manual Bromocresol Purple Method)
This test measures the distribution of different protein types in your blood serum using a manual method with bromocresol purple dye. It helps identify patterns of protein separation that may indicate conditions like infections, inflammation, or certain blood disorders.
["This test provides detailed insights into your blood proteins, useful for diagnosing and monitoring conditions affecting protein balance."]
Key facts at a glance
- LOINC
- 6743-9
- Short code
- WBC # Fld Manual
- Synonyms
- WBC # Fld Manual
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 4.5
- Ref high
- 11
- Critical range
- 2 N/A – 30 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- manual-count
- CPT
- 85004
- SNOMED
- 900000000000003002
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
The test separates blood proteins into distinct fractions—such as albumin, alpha-globulins, beta-globulins, and gamma-globulins—using electrophoresis. Bromocresol purple dye helps visualize these fractions by reacting with different proteins. This manual method allows for precise measurement of protein concentration in each fraction.
Why It’s Done
Doctors may order this test to diagnose or monitor conditions like multiple myeloma, Waldenström macroglobulinemia, liver disease, or chronic infections. It can also help assess protein abnormalities in conditions like nephrotic syndrome or certain autoimmune diseases.
Sample Preparation
A blood sample is drawn, and the serum (liquid part after clotting) is separated. The serum is then applied to a gel and subjected to an electric current to separate proteins based on size and charge. Bromocresol purple dye binds to proteins, creating visible bands that represent different protein groups.
Interpreting Results
Results show the relative amounts of each protein fraction. Abnormal patterns (e.g., elevated gamma-globulins, low albumin) may signal underlying health issues. Your healthcare provider will interpret results in the context of your symptoms and medical history.
Reference range
Adult: 4.5-11.0 x 10^9/LN/A
No special preparation required. Stay well hydrated.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Serum protein electrophoresis is critical for identifying conditions that alter protein distribution. For example, elevated gamma-globulins may indicate monoclonal gammopathy (e.g., multiple myeloma), while low albumin could signal liver disease or kidney problems. It’s often used alongside other tests to guide treatment decisions.
Short name
Manual Protein Electrophoresis
Frequently asked questions
What does a protein electrophoresis test show?
How long does it take to get results?
Can I eat or drink before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy