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Bromocresol Purple Protein Electrophoresis (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures protein distribution in your blood serum using a dye called bromocresol purple to separate and analyze different protein fractions, often used to evaluate conditions affecting protein balance.

Quick summary

["This test helps diagnose and monitor protein disorders, including those linked to kidney disease or liver conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57846-8
Short code
WBC # Pcar Auto
Synonyms
WBC # Pcar Auto
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
4.5
Ref high
11
Critical range
2 N/A – 30 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
automated-count
CPT
85004
SNOMED
900000000000003002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The bromocresol purple dye binds to proteins in your blood serum, allowing for separation into distinct bands. Each band corresponds to a specific protein group, including albumin and globulins, which can shift with certain medical conditions.

Why It’s Ordered

Healthcare providers may order this test if you have symptoms of protein-related disorders, such as swelling, unexplained weight loss, or signs of organ dysfunction (e.g., kidneys or liver). It’s also used to screen for conditions like multiple myeloma or nephrotic syndrome.

Common Conditions Monitored

This test helps track changes in protein levels that may indicate progression or response to treatment in conditions like chronic kidney disease, liver disease, or certain cancers.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in your arm. The serum portion is separated and prepared for analysis using the bromocresol purple dye method.

Reference range

Adult: 4.5-11.0 x 10^9/LN/A

Specimen: serum

No special preparation required. Stay well hydrated.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Abnormal protein patterns can signal underlying issues such as protein loss (e.g., in kidney disease), increased protein production (e.g., in myeloma), or metabolic imbalances. Changes in albumin or globulin levels may also reflect inflammation, infection, or nutritional deficiencies.

Short name

BCP Protein Electrophoresis

Frequently asked questions

What does a high or low protein band mean?
High levels in specific bands may indicate conditions like multiple myeloma (excess monoclonal proteins) or liver disease (altered globulin distribution). Low albumin levels often point to kidney disease or malnutrition, while abnormal globulin patterns may suggest chronic inflammation or autoimmune disorders.
How often should I get this test?
Your doctor will determine the frequency based on your medical history, symptoms, and treatment plan. Regular monitoring may be needed if you have chronic conditions like kidney disease or liver issues.
Can diet affect this test results?
Dietary changes, such as high protein intake or certain medications, may influence protein levels. Your healthcare provider may advise specific dietary restrictions or timing of the test for accuracy.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy