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Serum or Plasma Immunoassay for Anti-Nuclear Antibodies (ANA) by Immunofluorescence

Published: August 9, 2026Updated: August 16, 2026

This test measures antibodies your immune system may produce against parts of your own cells, often linked to autoimmune conditions.

Quick summary

["Helps identify potential autoimmune diseases by detecting unusual antibodies in your blood."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
41211-4
Short code
WNV IgG+IgM Ser Ql
Synonyms
WNV IgG+IgM Ser Ql
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is an ANA Test Used For?

ANA tests are primarily used to screen for autoimmune diseases, such as lupus, rheumatoid arthritis, or other connective tissue disorders. They don’t diagnose these conditions alone but can guide further testing if results are positive.

Why Might You Need This Test?

Your healthcare provider may order an ANA test if you have symptoms like joint pain, fatigue, rash, or unexplained organ issues. It’s also used in monitoring autoimmune diseases once diagnosed.

How Is the Test Performed?

A blood sample is taken from a vein in your arm. The serum or plasma is then tested for specific antibodies against nuclear components of cells using a fluorescent microscope.

What Do Results Mean?

Results are reported as either positive or negative. A positive result suggests the presence of ANA but doesn’t confirm an autoimmune disease. Follow-up tests may be needed for further evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: ANA tests play a critical role in diagnosing and managing autoimmune diseases. While many people with positive ANA results don’t have an autoimmune condition, they may indicate a higher risk. Timely evaluation by a healthcare provider is essential to determine the next steps, such as additional testing or treatment planning.

Short name

ANA (Serum/Plasma)

Frequently asked questions

What does a positive ANA test mean for me?
A positive ANA test means your blood contains antibodies that target parts of your cells. This doesn’t always mean you have an autoimmune disease, but it may warrant further testing to explore potential causes of your symptoms.
Can lifestyle changes affect ANA test results?
Lifestyle factors like diet, stress, or infections can sometimes influence immune activity, but they don’t typically change ANA test results permanently. Your healthcare provider will consider overall clinical context.
How often should I get retested if my ANA test is positive?
Retesting frequency depends on your symptoms and healthcare provider’s recommendations. Some people with positive ANA results are monitored regularly, while others may only need occasional checks if stable.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy