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Serum or plasma concentration of free fatty acids (FFA) by high-performance liquid chromatography (HPLC)

Published: August 9, 2026Updated: August 16, 2026

This test measures the levels of free fatty acids in your blood, which are important for energy production and other metabolic processes.

Quick summary

["Understanding your free fatty acid levels can help assess how your body uses fat for fuel and energy."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
103418-0
Short code
XR Face 2V
Synonyms
XR Face 2V
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
xr
CPT
82670
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms related to metabolic disorders, such as unexplained weight changes, fatigue, or issues with fat metabolism. It could also be part of a broader evaluation for conditions like diabetes, thyroid disorders, or liver disease.

What to Expect Before the Test

No special preparation is typically required, but you may be asked to fast for a certain period before your blood is drawn, depending on your specific test instructions. Avoiding certain foods or medications for a few hours can help ensure accurate results.

During the Test Process

A healthcare professional will draw blood from a vein in your arm. You may feel a slight pinch, but the procedure usually takes just a few minutes. The blood sample will be sent to a lab for analysis using HPLC to measure free fatty acid levels.

What the Results Mean

Results will show the concentration of free fatty acids in your blood. While specific reference ranges can vary by lab, abnormal levels may indicate issues with fat metabolism, hormone imbalances, or other metabolic conditions. Your provider will discuss these results with you in detail.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Free Fatty Acids (FFA) Profile

Frequently asked questions

What does my free fatty acid level tell me about my health?
Elevated levels might suggest conditions like hyperlipidemia, diabetes, or thyroid dysfunction, while lower levels could indicate malabsorption issues or certain metabolic syndromes. Your provider will interpret these results alongside your overall health and symptoms to guide further care.
How often should I get this test?
This test is usually ordered as needed based on your symptoms or medical needs. Your healthcare provider will determine the frequency based on your specific situation and ongoing health monitoring.
Can diet or lifestyle changes affect my FFA levels?
Yes, diet, exercise, and certain medications can influence free fatty acid levels. Avoiding high-fat foods for a few days before testing and maintaining a consistent lifestyle can help ensure more reliable results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy