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Vaginal Yeast and Pseudohyphae, Qualitative — KOH Preparation

Published: August 9, 2026Updated: August 17, 2026

A microscopic examination of vaginal fluid that looks for yeast and pseudohyphae, which may be associated with a vaginal yeast infection.

Quick summary

["This test looks for fungal forms in vaginal fluid that can support a diagnosis of vaginal candidiasis."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
55312-3
Short code
Yeast pseudohyphae Vag Ql KOH Prep
Synonyms
Yeast pseudohyphae Vag Ql KOH Prep
Category
liver
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
10
Ref high
40
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
koh-preparation
CPT
84484
SNOMED
302774002
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What is this test?

This test examines a sample of vaginal fluid for yeast and pseudohyphae. These fungal forms may be seen with vulvovaginal candidiasis, commonly called a vaginal yeast infection.

Why might I need this test?

A clinician may order it when there is vaginal itching, burning, redness, soreness, or an unusual discharge. It can help distinguish a yeast infection from other causes of vaginal symptoms.

How is the test performed?

A clinician collects vaginal fluid and prepares it with potassium hydroxide, or KOH. The sample is viewed under a microscope to check for yeast and pseudohyphae, and the result is reported as detected or not detected.

How do I prepare for the test?

Follow the clinician's collection instructions. Tell the clinician about any vaginal creams, suppositories, or douches used recently, because these products may affect the sample.

Reference range

Adult: 10-40 U/LN/A

Specimen: serum

No special preparation required. Avoid fatty meals before the test if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Finding yeast or pseudohyphae may support vaginal candidiasis, particularly when symptoms are typical. Yeast can occasionally be present without causing symptoms, so results should be interpreted with the examination and clinical history. A negative result does not completely rule out infection if symptoms remain concerning.

Short name

Vaginal yeast and pseudohyphae (KOH prep)

Frequently asked questions

What does a positive result mean?
A positive result means yeast, pseudohyphae, or both were seen in the vaginal sample. This is most consistent with a vaginal yeast infection when it matches your symptoms.
Does this test identify the exact Candida species?
No. A KOH preparation generally shows whether fungal forms are present but usually does not identify the specific Candida species. A culture or other fungal testing may be needed in certain cases.
Can I use vaginal medication before the test?
Ask your clinician whether to pause vaginal creams, suppositories, or douches, and for how long. These treatments can reduce the number of organisms found and make the result less reliable.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy