Serum/Plasma Protein Electrophoresis (Qualitative) with Bromocresol Purple Staining

What This Test Measures

Protein electrophoresis separates blood proteins into fractions (e.g., albumin, globulins) based on size and charge. Bromocresol purple staining highlights specific protein bands, allowing for qualitative comparison against normal patterns. This method helps identify shifts in protein composition that may not be detected by standard blood tests.

Why It’s Done

Your healthcare provider may order this test if you have symptoms like unexplained weight loss, fatigue, or swollen lymph nodes. It’s also used to monitor conditions like multiple myeloma, amyloidosis, or chronic inflammatory diseases where protein abnormalities are suspected.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in the arm. The serum or plasma is then processed and stained with bromocresol purple to visualize protein bands under an electrophoresis machine. Results are interpreted qualitatively by comparing patterns to standard references.

What to Expect Before the Test

No special preparation is needed, but inform your provider about medications (e.g., steroids) or recent illnesses that could affect protein levels. Avoid heavy meals 1–2 hours before the draw to ensure accurate blood collection.