Blotting analysis for specific protein variants (e.g., Alpha-1 Antitrypsin deficiency screening via immunoblot)
This test checks for variations in Alpha-1 Antitrypsin, a protein that protects lung and liver tissues from damage. It helps identify potential deficiencies that could increase your risk for lung diseases or liver problems.
["This test helps detect genetic mutations linked to Alpha-1 Antitrypsin deficiency, which can affect lung and liver health."]
Key facts at a glance
- LOINC
- 83002-6
- Short code
- Zic4 IgG SerPl Ql Line blot
- Synonyms
- Zic4 IgG SerPl Ql Line blot
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- CPT
- 82784
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Might Be Ordered
Your doctor may recommend this test if you have symptoms like chronic cough, wheezing, or liver-related issues, or if you have a family history of Alpha-1 Antitrypsin deficiency. It’s also used in screening for inherited conditions that affect protein production.
What to Expect During the Test
A blood sample is drawn from a vein in your arm. The blood is processed to separate proteins, which are then analyzed using an immunoblot technique. This test does not require fasting and typically takes place in a lab setting.
How the Results Are Interpreted
Results show the presence and pattern of Alpha-1 Antitrypsin protein bands. Abnormal patterns may indicate mutations or deficiencies that need further evaluation, such as genetic testing or additional clinical assessment.
Preparation and Sample Collection
No special preparation is needed. A simple blood draw is all that’s required. The sample is sent to a lab for analysis, and results are usually available within a few days to a week, depending on lab processes.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Alpha-1 Antitrypsin deficiency can lead to lung diseases (like emphysema) or liver cirrhosis. Early detection through this test allows for monitoring, lifestyle adjustments, or potential treatments to manage symptoms and complications.
Short name
Alpha-1 Antitrypsin (A1AT) Protein Profile (Immunoblot)
Frequently asked questions
What Does This Test Measure?
How Often Should I Get This Test?
Can I Eat or Drink Before This Test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy