Understanding Your Blood Test: What This Laboratory Test Measures and Why It Is Important
This test measures the amount of a specific DHEA metabolite in your urine collected over 24 hours, helping evaluate adrenal gland function.
{"Measures a DHEA metabolite in 24-hour urine collection","Helps assess adrenal gland and hormonal balance","Requires complete urine collection for accurate results","Used to diagnose certain hormonal disorders","Sample collected over 24 hours at home"}
Key facts at a glance
- LOINC
- 74505-9
- Short code
- 16-Beta,18-DiOH-DHEA 24h Ur
- Synonyms
- 16B,18-diOH-DHEA 24h Ur-sRate
- Category
- chemistry
- Sample
- urine
- Container
- 24-hour urine collection container (plastic, graduated)
- Units
- N/A
- Ref low
- 0.5
- Ref high
- 2.5
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–100 years
- Method
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
- CPT
- 82626
- SNOMED
- 271577006
- Specimen volume
- Complete 24-hour collection, record total volume
- Stability
- Refrigerated (2-8°C): 7 days; Frozen (-20°C): 30 days
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 72–168 hours
- Order
- routine
This test is a laboratory analysis of a small sample of your blood. It measures various components, such as red blood cells, white blood cells, platelets, hormones, or chemicals circulating in your blood. The results give your doctor valuable information about your overall health and can help detect infections, anemia, organ problems, and other conditions, often before you notice any symptoms.
The 16-Beta,18-dihydroxydehydroepiandrosterone test measures the amount of a specific metabolite (breakdown product) of dehydroepiandrosterone (DHEA) in your urine. DHEA is a hormone produced by your adrenal glands, which sit on top of your kidneys. This test uses a 24-hour urine collection because hormone levels can vary throughout the day, and a longer collection provides a more accurate picture of your body's hormone production.
The test is performed by drawing a small amount of blood, usually from a vein on the inside of your elbow or the back of your hand. A healthcare professional will clean the skin, wrap a band around your upper arm, and insert a thin, sterile needle to collect the sample into a tube. The procedure usually takes only a few minutes, and afterward a bandage is placed over the site. The sample is then sent to a laboratory, where a technologist analyzes it and reports the results to your doctor.
You will receive a special container from your healthcare provider. For 24 hours, you will collect all your urine in the container. This includes the first urine when you wake up on day one, but you exclude the first urine on day two. The container should be kept refrigerated during the collection period. After 24 hours, you return the complete container to the lab for analysis.
Your doctor may order this test as part of a routine checkup, to help diagnose the cause of symptoms you are having, or to monitor an ongoing condition and how well your treatment is working. The results can reveal signs of infection, anemia, clotting problems, kidney or liver issues, or nutritional deficiencies, and they help guide decisions about further testing, medications, or lifestyle changes needed to protect your health.
Your doctor may order this test if you have symptoms suggesting a hormonal imbalance, such as unusual hair growth, menstrual irregularities, infertility, or adrenal gland disorders. It helps doctors understand how well your adrenal glands are functioning and whether your body is processing DHEA properly.
Reference range
0.5-2.5 moles/24 hours (adults)N/A
Collect all urine over a 24-hour period. Discard the first morning void and begin collection. Collect all subsequent voided urine including the first morning void of the second day. Record total volume. Keep container refrigerated during collection. Inform patient of collection start and end times.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test is primarily used to evaluate adrenal gland function and diagnose conditions affecting adrenal hormone production. Abnormal levels of 16β,18-DiOH-DHEA may indicate adrenal tumors, congenital adrenal hyperplasia, or other adrenal disorders. The test can also help distinguish between different types of hormonal disorders and guide treatment decisions. Results are interpreted in conjunction with other hormone tests and clinical symptoms.
Frequently asked questions
Do I need to fast before this test?
What happens if I miss a urine collection during the 24 hours?
Can I take my medications during the collection period?
How long will it take to get results?
Sources
- 1
- 2
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy