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16b18diohdheaurscnc

Published: August 9, 2026Updated: August 16, 2026

This urine test measures the level of 16-beta,18-dihydroxydehydroepiandrosterone, a hormone metabolite that helps evaluate adrenal gland function.

Quick summary

{"Measures a specific hormone metabolite in urine","Helps diagnose adrenal gland disorders","Involves collecting a urine sample over a specific time period","Results help doctors evaluate hormone-producing conditions","No special preparation is typically required"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
74503-4
Short code
16β,18-diOH-DHEA
Synonyms
16B,18-diOH-DHEA Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container, 24-hour urine collection container with preservative if needed
Units
N/A
Ref low
0.5
Ref high
5
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT
82540
SNOMED
314256001
Specimen volume
Minimum 10 mL; preferably complete 24-hour collection (typical volume 1000-2000 mL)
Stability
Room temperature: 24-48 hours; Refrigerated (2-8°C): 5-7 days; Frozen (-20°C or below): 2-3 months
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
72–168 hours
Order
routine

What is this test?

The 16-beta,18-dihydroxydehydroepiandrosterone (16β,18-DiOH-DHEA) test measures the amount of this specific hormone metabolite in your urine. This substance is produced when your body breaks down dehydroepiandrosterone (DHEA), a hormone made by your adrenal glands. The test helps doctors understand how well your adrenal glands are functioning and can aid in detecting certain hormonal disorders.

Why is this test performed?

Your doctor may order this test to help diagnose or monitor conditions affecting your adrenal glands, such as adrenal tumors, congenital adrenal hyperplasia, or other disorders that affect hormone production. It may also be used to evaluate unusual symptoms related to hormone imbalances, such as excessive hair growth, early puberty, or adrenal insufficiency.

How is the sample collected?

For this test, you will typically collect all urine produced during a 24-hour period. You will be given a special container to collect your urine. During the collection period, keep the container refrigerated or on ice. Make sure to label the container with your name, date, and the start and end times of collection. Follow your healthcare provider's instructions carefully to ensure accurate results.

What do the results mean?

Abnormal levels of 16β,18-DiOH-DHEA may indicate adrenal gland problems. Your doctor will interpret your results along with other tests and your clinical symptoms to make a diagnosis. Do not try to interpret your results on your own—always discuss them with your healthcare provider who can explain what the numbers mean for your specific situation.

Are there any risks?

This test involves only normal urine collection, so there are no significant physical risks. Some people may find the 24-hour collection process inconvenient. Make sure to follow proper hygiene practices during collection to avoid contamination of the sample.

Reference range

Reference range varies by laboratory method and population. Typical 24-hour urinary excretion: 0.5-5.0 nmol/24h. Consult laboratory-specific reference intervals.N/A

Specimen: urine

Collect urine in a sterile container. Avoid contamination with blood or menstrual fluid. Inform the laboratory of any medications or supplements, particularly steroid hormones or adrenal extracts. No specific dietary restrictions required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Do I need to fast before this test?
Typically, no fasting is required for this urine test. However, follow any specific instructions given by your healthcare provider. It is important to stay well-hydrated during the collection period unless otherwise directed.
Can I collect the urine sample at home?
Yes, this test is performed using a 24-hour urine collection kit that you take home. You will receive detailed instructions from your healthcare provider or laboratory on how to collect and store the sample properly.
Should I stop taking medications before the test?
Inform your doctor about all medications, supplements, and vitamins you are taking. Some medications may affect hormone levels. Do not stop any prescribed medications without consulting your healthcare provider first.
How long does it take to get results?
Results typically take a few days to a week, depending on the laboratory. Your healthcare provider will contact you with the results or discuss them at your next appointment.
What happens if my results are abnormal?
An abnormal result does not necessarily mean you have a serious condition. Your doctor will consider your symptoms, medical history, and other test results to determine what, if any, follow-up testing or treatment is needed.
  1. 1
    MedlinePlus - Adrenal Gland FunctionSource
  2. 2
    Mayo Clinic - Hormone TestsSource
  3. 3
    LOINC - 74503-4Source

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy