2425oh2vitdserplscnc

Published: August 9, 2026Updated: August 10, 2026

This test measures the levels of 24,25-dihydroxyvitamin D3 and D2, which are breakdown products of vitamin D, in your blood to evaluate vitamin D metabolism and bone health.

Quick summary

{"The test measures 24,25-dihydroxyvitamin D, a key metabolite that indicates how your body processes vitamin D","It helps doctors assess kidney function and vitamin D status","Abnormal levels may indicate kidney disease, bone disorders, or vitamin D deficiency","A blood sample is taken from a vein in your arm","Results help guide treatment for bone health and calcium balance"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 10, 2026Editorial policy

Key facts at a glance

LOINC: 106774-3
Short code: 24,25-DHVD
Synonyms: 24,25(OH)2 VitD SerPl-sCnc
Category: chemistry
Sample: serum
Container: Red-top serum separator tube (SST) or gold-top tiger-top tube
Units: N/A
Ref low: 0.9
Ref high: 3.8
Fasting: false
Population: adult
Sex: all
Age range: ≥ 18 years
Method: Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
CPT: 82306 or 82652 (depends on methodology)
SNOMED: 314255006
Specimen volume: 1.0 mL of serum minimum, 2.0 mL preferred
Stability: Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C or below): 30 days. Protect from light.
Hemolysis max: 50
Lipemia max: 60
Icterus max: 40
TAT: 72–168 hours
Order: routine

What is this test?

The 24,25-dihydroxyvitamin D test measures two forms of vitamin D metabolites in your blood: 24,25-dihydroxyvitamin D3 (from animal-based vitamin D3) and 24,25-dihydroxyvitamin D2 (from plant-based vitamin D2). These are intermediate products created when your body breaks down vitamin D. The test is performed on a sample of blood taken from a vein in your arm.

Why is this test done?

This test is primarily used to evaluate how well your kidneys are functioning, as the conversion of vitamin D to 24,25-dihydroxyvitamin D occurs in the kidneys. It also helps assess vitamin D status, bone metabolism, and conditions affecting calcium balance such as rickets, osteomalacia, kidney disease, and certain parathyroid disorders.

How should I prepare?

No special preparation is typically needed for this test. You may be asked to fast for several hours before the blood draw in some cases. Always follow your healthcare provider's specific instructions. Inform your doctor about any medications, supplements, or vitamins you are taking, as these may affect vitamin D levels.

What do the results mean?

Normal 24,25-dihydroxyvitamin D levels indicate healthy vitamin D metabolism and kidney function. Low levels may suggest kidney disease, severe vitamin D deficiency, or problems with vitamin D absorption. Your doctor will interpret your results alongside other tests and your overall health condition.

Reference range

Reference range: 1.5-5.0 ng/mL (0.9-3.8 nmol/L) for 24,25-dihydroxyvitamin D. Optimal levels associated with bone health and normal calcium metabolism.N/A

Specimen: serum

No special preparation required. Patient does not need to fast. Avoid vitamin D supplements 24 hours prior to collection if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is fasting required before this test?

Fasting is not always required, but follow your healthcare provider's instructions. Some laboratories may request fasting for 8-12 hours. Your doctor will inform you of any specific preparation needed.

Can I take my vitamins or supplements before the test?

Inform your doctor about all supplements and medications you are taking. Vitamin D supplements can affect test results. Your doctor may advise you to stop taking vitamin D supplements before the test.

What conditions cause abnormal 24,25-dihydroxyvitamin D levels?

Low levels may be seen in chronic kidney disease, severe vitamin D deficiency, liver disease, and certain genetic disorders. High levels may occur with excessive vitamin D supplementation or certain conditions affecting vitamin D metabolism.

How is this test different from a regular vitamin D test?

Regular vitamin D tests typically measure 25-hydroxyvitamin D, the major circulating form of vitamin D. The 24,25-dihydroxyvitamin D test measures a specific metabolite that provides additional information about kidney function and vitamin D metabolism.

Will I need this test repeated?

Your doctor will determine if repeat testing is needed based on your initial results and clinical condition. Repeat testing may be recommended to monitor treatment effectiveness or disease progression.

Sources

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 10, 2026. · Read our editorial policy