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25hexanedioneurmcnc

Published: August 9, 2026Updated: August 16, 2026

This test measures 2,5-Hexanedione in urine, a biomarker used to detect exposure to n-hexane, a toxic industrial solvent.

Quick summary

{"2,5-Hexanedione is a breakdown product of n-hexane, a common industrial solvent","This test helps monitor occupational exposure in workers using n-hexane products","Elevated levels may indicate n-hexane poisoning, which can affect the nervous system","The test is performed on a urine sample collected over 24 hours or as a spot sample","Regular testing is recommended for workers in high-risk industries like printing and shoe manufacturing"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
31170-4
Short code
2,5-Hex
Synonyms
2,5Hexanedione Ur-mCnc
Category
chemistry
Sample
urine
Container
Clean, labeled urine container (sterile plastic container with screw cap)
Units
N/A
Ref low
0
Ref high
0.5
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Gas Chromatography-Mass Spectrometry (GC-MS) or Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT
82520
SNOMED
313272009
Specimen volume
Minimum 10 mL, preferably 20-50 mL
Stability
Refrigerate at 2-8°C if analysis within 7 days; Freeze at -20°C or below for longer storage; Protect from light
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
24–72 hours
Order
routine

What is this test?

The 2,5-Hexanedione urine test measures the amount of 2,5-Hexanedione in your urine. 2,5-Hexanedione is a chemical that your body produces when it breaks down n-hexane, a solvent widely used in industrial settings. This test is primarily used for occupational health monitoring to check if workers have been exposed to dangerous levels of n-hexane.

Why is it important?

N-hexane is commonly used in industries such as printing, shoe manufacturing, petroleum refining, and adhesives production. Exposure to high levels of n-hexane can cause serious health problems, particularly damage to the peripheral nervous system (peripheral neuropathy). Early detection of exposure through this test can help prevent long-term nerve damage.

How is the test performed?

The test requires a urine sample. Your healthcare provider may request either a spot urine sample (single collection) or a 24-hour urine collection for more accurate results. The sample is then sent to a laboratory where the concentration of 2,5-Hexanedione is measured. No special preparation is typically needed, but follow your healthcare provider's specific instructions.

What do the results mean?

Results are typically reported as milligrams of 2,5-Hexanedione per liter of urine (mg/L) or as a ratio to creatinine. Normal (background) levels may vary depending on environmental exposure. Elevated levels indicate significant occupational exposure to n-hexane. Your occupational health department or healthcare provider will interpret your results based on your work history and potential exposure levels.

Reference range

Less than 0.5 mg/L (or as per laboratory reference range); Non-exposed individuals typically have undetectable levelsN/A

Specimen: urine

Collect urine at end of work shift preferred; Avoid contamination of sample; Provide occupational exposure history

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Who should have this test?
This test is recommended for workers in industries where n-hexane is commonly used, including printing, shoe manufacturing, furniture making, petroleum refining, and adhesive production. Regular monitoring is especially important for workers with potential for high or prolonged exposure.
What are the symptoms of n-hexane exposure?
Early symptoms of n-hexane exposure may include headache, dizziness, and fatigue. As exposure continues, symptoms can progress to peripheral neuropathy, causing numbness, tingling, muscle weakness, and loss of coordination in the hands and feet. In severe cases, muscle atrophy and nerve damage may become irreversible.
How can I reduce my exposure to n-hexane?
Workplace exposure can be reduced through proper ventilation, using personal protective equipment (such as gloves and respirators), following safety protocols, and ensuring regular occupational health monitoring. If you work with n-hexane-containing products, always follow your employer's safety guidelines and report any symptoms of exposure to your supervisor immediately.
Is the test painful or dangerous?
No, the test is simple and safe. It only requires collecting a urine sample, which is non-invasive and carries no health risks. The procedure is the same as a routine urinalysis.
  1. 1
    ATSDR - Toxic Substances Portal: n-HexaneSource
  2. 2
    OSHA - n-HexaneSource
  3. 3
    NIOSH - n-HexaneSource

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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy