: Fifth Most Predominant Hemoglobin by Electrophoresis
: This test identifies and measures the fifth most abundant hemoglobin fraction present in a blood sample using electrophoresis, a technique that separates hemoglobin types by their electrical charge.
[": The test helps characterize unusual or minor hemoglobin fractions that may indicate a hemoglobin variant or related blood condition."]
Key facts at a glance
- LOINC
- 64121-7
- Short code
- 5th most predominant Hb DBS
- Synonyms
- 5th most predominant Hb DBS
- Category
- hematology
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
: What This Test Measures
: Hemoglobin is the oxygen-carrying protein in red blood cells, and normally most hemoglobin is of the adult type. Electrophoresis separates the different hemoglobin types in your sample so the laboratory can see which fractions are present and roughly how much of each there is. This test focuses specifically on the fifth most predominant fraction detected.
: Why It Is Ordered
: Your healthcare provider may order this test when a routine hemoglobin evaluation shows an unexpected pattern or when there is suspicion of an inherited hemoglobin variant. Identifying a minor fraction can help explain findings such as an unusual red blood cell shape or an atypical screening result.
: How the Sample Is Collected
: A small blood sample is drawn from a vein, usually in your arm. The sample is then processed in the laboratory, where the hemoglobin types are separated by electrophoresis and the resulting pattern is examined.
: What the Results Mean
: Results describe which hemoglobin fraction appears as the fifth most abundant and how prominent it is relative to the others. Because normal patterns vary and interpretation depends on the full hemoglobin profile, your provider will interpret this result alongside your other tests, symptoms, and history rather than in isolation.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
: Identifying minor hemoglobin fractions can support the diagnosis of hemoglobin variants such as thalassemias or structural hemoglobin abnormalities. However, a single fraction result is rarely conclusive on its own; it is interpreted together with the complete electrophoresis pattern, blood counts, and clinical context, and abnormal findings typically prompt confirmatory testing.
Short name
: Hemoglobin Fraction (5th Most Predominant)
Frequently asked questions
: Is this test done on its own?
: Does an unusual result always mean I have a disease?
: Will I need to fast or prepare for this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy