Skip to content

: Fifth Most Predominant Hemoglobin by Electrophoresis

Published: August 9, 2026Updated: August 16, 2026

: This test identifies and measures the fifth most abundant hemoglobin fraction present in a blood sample using electrophoresis, a technique that separates hemoglobin types by their electrical charge.

Quick summary

[": The test helps characterize unusual or minor hemoglobin fractions that may indicate a hemoglobin variant or related blood condition."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
64121-7
Short code
5th most predominant Hb DBS
Synonyms
5th most predominant Hb DBS
Category
hematology
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

: What This Test Measures

: Hemoglobin is the oxygen-carrying protein in red blood cells, and normally most hemoglobin is of the adult type. Electrophoresis separates the different hemoglobin types in your sample so the laboratory can see which fractions are present and roughly how much of each there is. This test focuses specifically on the fifth most predominant fraction detected.

: Why It Is Ordered

: Your healthcare provider may order this test when a routine hemoglobin evaluation shows an unexpected pattern or when there is suspicion of an inherited hemoglobin variant. Identifying a minor fraction can help explain findings such as an unusual red blood cell shape or an atypical screening result.

: How the Sample Is Collected

: A small blood sample is drawn from a vein, usually in your arm. The sample is then processed in the laboratory, where the hemoglobin types are separated by electrophoresis and the resulting pattern is examined.

: What the Results Mean

: Results describe which hemoglobin fraction appears as the fifth most abundant and how prominent it is relative to the others. Because normal patterns vary and interpretation depends on the full hemoglobin profile, your provider will interpret this result alongside your other tests, symptoms, and history rather than in isolation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

: Identifying minor hemoglobin fractions can support the diagnosis of hemoglobin variants such as thalassemias or structural hemoglobin abnormalities. However, a single fraction result is rarely conclusive on its own; it is interpreted together with the complete electrophoresis pattern, blood counts, and clinical context, and abnormal findings typically prompt confirmatory testing.

Short name

: Hemoglobin Fraction (5th Most Predominant)

Frequently asked questions

: Is this test done on its own?
: Usually not. It is generally part of a broader hemoglobin electrophoresis evaluation that examines all the hemoglobin fractions together, and this specific fraction is interpreted within that overall pattern.
: Does an unusual result always mean I have a disease?
: No. Some minor hemoglobin fractions can be found in people without significant health problems. Your provider will consider the full picture, including other test results and your medical history, before drawing conclusions.
: Will I need to fast or prepare for this test?
: Fasting is typically not required for hemoglobin electrophoresis. Follow any specific instructions your healthcare provider or laboratory gives you, and let them know about any recent blood transfusions, since these can affect results.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy