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6-Alpha-Hydroxytestosterone, 24-Hour Urine — Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

A 24-hour urine test that measures 6-alpha-hydroxytestosterone, a steroid metabolite related to androgen production and metabolism.

Quick summary

["This test helps assess steroid metabolism and androgen production when interpreted with the rest of your endocrine evaluation."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57599-3
Short code
6A-OH-THS 2D p high Dex 24h Ur-mCnc
Synonyms
6A-OH-THS 2D p high Dex 24h Ur-mCnc
Category
liver
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures 6-alpha-hydroxytestosterone in urine collected for 24 hours. It is a specialized steroid test that may be used to evaluate androgen production and metabolism.

Why might I need this test?

A clinician may order this test when investigating an unusual androgen or adrenal steroid pattern, particularly when symptoms or other laboratory findings suggest a hormonal imbalance. It is generally interpreted together with other hormone tests.

How is the test performed?

You collect all urine for a full 24-hour period in the container provided by the laboratory. The collected urine is then analyzed to determine the concentration of 6-alpha-hydroxytestosterone.

How do I prepare for the test?

Follow the laboratory's collection instructions, including the starting and ending times and how to store the container. Do not begin the collection until you are ready to collect every urine sample during the 24-hour period.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine 6-Alpha-Hydroxytestosterone

Frequently asked questions

Does this test require a fasting blood draw?
No. This test uses urine collected over 24 hours, not a fasting blood sample. Follow the specific instructions supplied by the laboratory.
What happens if I miss part of the urine collection?
The result may be inaccurate or the collection may need to be repeated. Contact the laboratory for guidance if you miss a sample or the collection period is not exactly 24 hours.
Does one abnormal result diagnose a hormone disorder?
No. Your clinician will consider the result alongside your symptoms, examination, medications, and other laboratory tests to determine its meaning.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy