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6-Alpha-Hydroxytestosterone, 24-Hour Urine — Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

Measures the amount of 6-alpha-hydroxytestosterone, a steroid metabolite, collected over 24 hours to help evaluate steroid metabolism and adrenal function.

Quick summary

["This 24-hour urine test measures a steroid metabolite that may help assess adrenal steroid production and metabolism."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
57597-7
Short code
6A-OH-THS pre hi Dex 24h Ur-mCnc
Synonyms
6A-OH-THS pre hi Dex 24h Ur-mCnc
Category
liver
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures 6-alpha-hydroxytestosterone in urine collected over 24 hours. It is a specialized steroid-metabolite test used to help evaluate adrenal hormone production and the body's handling of steroid hormones.

Why might I need this test?

A clinician may order this test when evaluating an adrenal disorder, unusual steroid laboratory results, or symptoms that could be related to abnormal hormone production. It is usually interpreted together with other hormone and steroid tests.

How is the test performed?

You collect all urine for a full 24-hour period, beginning and ending at the specified times. The laboratory measures the amount of 6-alpha-hydroxytestosterone in the collection and adjusts for the total amount of urine collected.

How do I prepare for the test?

Follow the collection container and timing instructions provided by the laboratory, and note any urine that is missed. Keep the container refrigerated if instructed, bring it to the laboratory promptly, and ask your clinician before changing medicines or supplements.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine 6-Alpha-Hydroxytestosterone

Frequently asked questions

Do I need to fast for this test?
Fasting is generally not required, but follow any specific instructions from the laboratory or ordering clinician. Medicine and supplement use should be reported because they can affect steroid measurements.
What happens if I miss part of the urine collection?
Tell the laboratory and your clinician, because a missed collection can make the measured amount appear lower or otherwise inaccurate. The collection time and any missed portions may need to be documented.
Is one abnormal result enough to diagnose a condition?
No. This test is usually interpreted with other findings and repeat or confirmatory testing. Your clinician can explain what the result means in the context of your symptoms and medical history.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy