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Newborn Screening Test (Heel Stick Blood Test for Infants)

Published: August 9, 2026Updated: August 16, 2026

A blood test that determines an infant's ABO blood type (A, B, AB, or O) during the first year of life.

Quick summary

{"The test identifies your baby's blood type as A, B, AB, or O","A small blood sample is taken from the baby's heel or arm","Results help ensure safe blood transfusions if needed","The test is important for managing jaundice and potential blood disorders","Knowing the blood type is useful for future medical care"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
93905-8
Short code
ABO-inf
Synonyms
ABO Group infant Bld
Category
chemistry
Sample
whole-blood
Container
EDTA tube (lavender/purple top) or cord blood collection tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
0–1 years
Method
Tube agglutination, gel column assay, or automated immunohematology analyzer
CPT
86803
SNOMED
365679008
Specimen volume
1-3 mL whole blood; minimum 0.5 mL for pediatric/infant collection
Stability
Room temperature: 72 hours; Refrigerated (2-8°C): 7 days; Do not freeze
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
1–24 hours
Order
routine

This test is a newborn screening blood test that checks your baby for rare but serious health conditions, including metabolic disorders, hormone problems, and certain inherited diseases, often before any signs or symptoms appear.

The ABO blood type test is a simple blood test that determines which of the four ABO blood types your infant has: A, B, AB, or O. This test identifies the specific antigens (markers) on the surface of red blood cells. The ABO system is the most important blood group system for blood transfusions and pregnancy compatibility.

Babies are screened shortly after birth because many of these conditions cause no visible problems at first. Early detection allows treatment to begin right away, which can prevent serious complications, developmental delays, or life-threatening illness.

This test may be performed for several reasons during infancy: (1) To determine blood type for medical records, (2) To help diagnose and manage jaundice, especially when there is a mismatch between mother's and baby's blood types, (3) To prepare for potential blood transfusions if needed, (4) As part of routine newborn screening in some hospitals, (5) To identify infants at risk for ABO incompatibility, which can cause jaundice in the first days of life.

A health care provider cleans your baby's heel, makes a small prick with a sterile lancet, and collects a few drops of blood on a special filter paper card. The sample is sent to a laboratory for analysis, usually when your baby is 24 to 48 hours old.

A healthcare professional will clean the area (usually the heel for newborns or the arm for older infants) with an antiseptic. A small needle or lancet will be used to collect a few drops of blood. The sample is then sent to a laboratory where it is mixed with special antibodies to determine the blood type. The test involves mixing the blood with reagents containing anti-A and anti-B antibodies and observing how the blood cells react.

Your baby may feel a quick sting or pinch when the heel is pricked, but the discomfort is brief and mild. Most babies cry for only a moment and settle down quickly, especially when held, comforted, or fed right after the procedure.

Your baby may feel a brief pinch or stinging sensation when the blood sample is taken. This usually lasts only a moment. Comfort techniques such as swaddling, feeding, or gentle rocking can help soothe your baby during and after the procedure.

This test is very safe. The most common effects are minor bruising, slight bleeding, or a small sore at the heel puncture site, which typically heal within a few days. Serious complications are extremely rare, and the benefits of early detection far outweigh the risks.

The risks are minimal and include slight bruising or soreness at the puncture site. In rare cases, bleeding or infection may occur. The healthcare provider will apply pressure to stop any bleeding and keep the area clean.

Reference range

Results reported as A, B, AB, or O. Expected ABO group varies based on parental blood types and inheritance patterns.N/A

Specimen: whole-blood

No special preparation required. Blood typing can be performed on cord blood at birth or via venous or capillary sample in infants.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

ABO blood type testing in infants is clinically significant for several reasons. First, it helps identify infants at risk for ABO hemolytic disease of the newborn (HDN), a condition where the mother's antibodies attack the baby's red blood cells, causing jaundice and anemia. Second, knowing the blood type is essential for safe blood transfusions should your baby ever need one. Third, it provides important information for healthcare providers to monitor and manage neonatal jaundice effectively. Fourth, the blood type information becomes part of your child's permanent medical record and is useful throughout their lifetime for transfusion safety, pregnancy planning, and emergency medical care.

Frequently asked questions

When will I get the results?
Results are typically available within a few hours to one day. Your healthcare provider will inform you of the results during a follow-up appointment or by phone.
Does my baby need to fast before the test?
No fasting is required for an ABO blood type test. You can feed your baby normally before and after the test.
What if my baby's blood type is different from mine?
This is completely normal and expected. Each person inherits their blood type from their parents, and it is common for a baby's blood type to differ from either parent's blood type.
Can the blood type change over time?
No, a person's ABO blood type is determined by their genes and does not change throughout their lifetime.
What happens if the test shows ABO incompatibility?
If ABO incompatibility is detected (usually between mother and baby), your healthcare team will monitor your baby more closely for signs of jaundice. Additional tests such as bilirubin levels may be ordered, and treatment such as phototherapy may be recommended if needed.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy