Newborn Screening Blood Test (Heel Stick)
A blood test that determines your baby's blood type (A, B, AB, or O) and Rh factor (positive or negative).
{"This test identifies your baby's blood type and Rh factor","It helps check for blood compatibility issues between mother and baby","Results are important for safe blood transfusions if needed","Rh-negative mothers may need special care during pregnancy","The test uses a small blood sample from your baby"}
Key facts at a glance
- LOINC
- 93906-6
- Short code
- ABO-Rh Infant
- Synonyms
- ABO + Rh infant Bld
- Category
- chemistry
- Sample
- whole-blood
- Container
- EDTA tube (lavender top/purple cap) - K2-EDTA or K3-EDTA
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–2 years
- Method
- Serum/plasma agglutination with anti-A, anti-B, anti-AB, anti-D (Rh) reagents; column agglutination technology (CAT) or tube method
- CPT
- 86900 (ABO blood typing); 86901 (Rh blood typing)
- SNOMED
- 365679006 (ABO blood group); 127778009 (Rh blood group); 169992004 (Blood typing during infancy)
- Specimen volume
- 3-5 mL whole blood (minimum 1 mL for pediatric tubes)
- Stability
- Room temperature (20-25°C): 72 hours; Refrigerated (2-8°C): up to 7 days. Do not freeze.
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 1–24 hours
- Order
- routine
This test is a simple blood screening done shortly after birth to check your baby for serious but rare health conditions that often cause no noticeable signs in the first days of life. Finding these conditions early means treatment can begin right away, which helps protect your baby's growth, development, and long-term health.
The ABO and Rh blood type test is a simple blood test that determines two important things about your baby's blood: (1) the ABO blood group (A, B, AB, or O) and (2) the Rh factor (Rh-positive or Rh-negative). This test is performed specifically during infancy to help identify potential blood type incompatibilities between the mother and baby that could cause health problems.
Babies are screened because many inherited and metabolic conditions do not cause obvious symptoms at first but can lead to developmental delays, organ damage, or life-threatening illness if they are not found and treated within the first days or weeks of life. Early detection through newborn screening gives your baby the best possible chance at a healthy start.
During infancy, this test is important for several reasons: (1) To check for ABO or Rh incompatibility between the mother and baby, which can cause jaundice or anemia in newborns; (2) To determine if a Rh-negative mother needs Rh immunoglobulin injections to prevent complications in future pregnancies; (3) To establish the baby's blood type for medical records; (4) To ensure safe blood transfusion if the baby ever needs one.
A health care provider will gently clean your baby's heel, make a small prick with a sterile lancet, and collect a few drops of blood onto a special filter paper card. Most babies feel only a brief pinch and may cry for a moment. The blood sample is then sent to a laboratory for testing, and the results are usually shared with your baby's doctor.
A healthcare provider will collect a small blood sample from your baby's heel (heel stick) or from a vein in the arm. The blood is then sent to a laboratory where it is mixed with special antibodies to determine how the red blood cells react. This helps identify the exact blood type and Rh factor. The procedure only takes a few minutes and is generally well-tolerated by infants.
This test is very safe. The most common effects are a brief sting during the heel prick, mild fussiness, or a small bruise where the blood was drawn, and these usually go away within a few days. Serious problems such as infection are extremely rare. Sometimes a repeat sample is requested, which does not necessarily mean your baby has a health condition.
The risks are minimal and include slight pain during the blood draw, possible bruising or swelling at the puncture site, and rarely, infection. The benefits of knowing your baby's blood type far outweigh these minor risks. Your healthcare provider will ensure the procedure is done safely and comfortably for your little one.
Reference range
Expected result: A, B, AB, or O; Rh: Positive or Negative. Reference range is not applicable as this is a qualitative test determining blood group phenotype.N/A
No special preparation required. For infant testing, ensure proper patient identification and maternal blood group documentation if available. No fasting required.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
ABO and Rh blood typing during infancy is clinically significant because it helps identify blood type incompatibility between mother and baby. If a mother is Rh-negative and her baby is Rh-positive, the mother's immune system may produce antibodies that can affect future pregnancies. Early detection allows doctors to take preventive measures. Additionally, ABO incompatibility can cause newborn jaundice, and knowing the blood type helps doctors monitor and treat this condition appropriately. This information is also crucial if the baby ever needs a blood transfusion.
Frequently asked questions
Does this test hurt my baby?
How long does it take to get results?
What if my baby has a different blood type than me?
Does my baby need to fast before this test?
Will my baby need more tests after this?
Sources
- 1
- 2Mayo Clinic - Blood TypeSource
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy