Skip to content

About This Test: What Patients Need to Know

Published: August 9, 2026Updated: August 10, 2026

This test checks for the presence of both acetaminophen (a pain reliever) and oxycodone (a strong pain medication) in your blood.

Quick summary

{"The test detects two pain medications: acetaminophen (Tylenol) and oxycodone (a prescription opioid)","It helps doctors monitor medication levels and ensure safe dosing","Blood is drawn from a vein in your arm for this test","Results help healthcare providers check for overdose or verify proper medication use"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 10, 2026Editorial policy

Key facts at a glance

LOINC
43719-4
Short code
ACET-OXY
Synonyms
Acetamin+oxyCODONE SerPl Ql
Category
chemistry
Sample
serum
Container
Serum Separator Tube (SST) or Plain Red Top Tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
Immunoassay (Chemiluminescent Microparticle Chemiflex)
CPT
80307
SNOMED
122461009
Specimen volume
1.0 mL minimum, 2.0 mL preferred
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 7 days; Frozen (-20°C or below): 30 days
Hemolysis max
50
Lipemia max
50
Icterus max
20
TAT
4–24 hours
Order
routine

This test is a diagnostic procedure that helps your healthcare provider gather important information about your health. It involves examining a sample, such as blood, urine, or tissue, or using imaging technology to evaluate how your body is functioning and to support an accurate diagnosis and treatment plan.

The Acetaminophen and Oxycodone blood test is a laboratory test that detects whether both medications are present in your bloodstream. Acetaminophen is found in over-the-counter pain relievers like Tylenol, while oxycodone is a prescription opioid medication used for moderate to severe pain management. This test can determine if these drugs are in your system and in some cases, measure how much is present.

Your doctor may order this test to confirm or rule out a suspected condition, monitor an ongoing illness, or check how well your current treatment is working. It can also be part of a routine checkup to screen for potential health problems before symptoms appear, allowing earlier care when needed.

Your healthcare provider may order this test for several reasons: to monitor your medication levels if you are taking both drugs, to check for accidental or intentional overdose, to ensure you are taking your medications as prescribed, or to evaluate symptoms of drug toxicity such as nausea, dizziness, or liver problems.

The test is usually performed by a trained healthcare professional in a clinic, hospital, or laboratory. Depending on the type of test, a small sample of blood may be drawn from a vein in your arm, or you may be asked to provide a urine or other sample. The procedure is typically quick, and most people can return to their normal activities right afterward.

A healthcare professional will draw a small sample of blood from a vein in your arm using a needle. The blood sample will be sent to a laboratory for analysis. The test requires a serum or plasma sample, which is obtained after the blood is processed in the lab. You do not need to fast before this test unless your doctor specifically instructs you to do so.

Your results will show whether the measured values fall within the normal reference range for this test. Results within the expected range usually suggest no apparent abnormality, while values that are higher or lower than normal may indicate a condition that requires further evaluation. Your healthcare provider will review the findings with you and explain what they mean for your health.

Results are typically reported as 'detected' or 'not detected,' or may show the specific concentration of each medication in your blood. Your doctor will interpret these results based on your medical history, the medications you are currently taking, and your symptoms. Having these medications in your system may be expected if you are under treatment, but unexpectedly high levels could indicate an overdose.

Reference range

Not Detected - This is a qualitative test for the presence of acetaminophen and oxycodone. Results are reported as Detected or Not Detected.N/A

Specimen: serum

No fasting required. Inform laboratory of all medications including over-the-counter pain relievers, prescription opioids, and supplements. Recent ingestion of acetaminophen-containing products should be disclosed.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

How long do these drugs stay in your blood?
Acetaminophen typically remains detectable in blood for about 24-48 hours after the last dose. Oxycodone can be detected for about 24-72 hours, depending on factors like metabolism, dosage, and frequency of use.
Will this test show if I'm taking too much medication?
Yes, this test can help determine if medication levels in your blood are higher than expected, which may indicate an overdose. However, the specific threshold for 'too much' varies based on individual factors and should be interpreted by your healthcare provider.
Do I need to stop taking my medications before the test?
No, you should continue taking your medications as prescribed by your doctor. Stopping suddenly may cause withdrawal symptoms or inadequate pain control. Always follow your healthcare provider's specific instructions.
Is the test painful?
You may feel a brief pinch or mild discomfort when the needle is inserted to draw blood. This is typically well-tolerated and the procedure only takes a few minutes.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 10, 2026. · Read our editorial policy