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Acetazolamidebldmcnc

Published: August 9, 2026Updated: August 15, 2026

This test measures the amount of acetazolamide, a carbonic anhydrase inhibitor medication, in your blood to ensure proper dosing and therapeutic effectiveness.

Quick summary

{"Measures acetazolamide concentration in whole blood samples","Helps doctors monitor drug levels for safety and effectiveness","Used to treat glaucoma, altitude sickness, epilepsy, and fluid retention","Ensures drug levels are within therapeutic range","May help adjust dosage to prevent side effects or toxicity"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 15, 2026Editorial policy

Key facts at a glance

LOINC
59598-3
Short code
ACET
Synonyms
acetaZOLAMIDE Bld-mCnc
Category
chemistry
Sample
whole-blood
Container
Lavender top (EDTA) tube or green top (heparin) tube for whole blood collection
Units
N/A
Ref low
5
Ref high
15
Critical range
— – 40 N/A
Fasting
false
Population
adult
Sex
all
Age range
0–120 years
Method
High Performance Liquid Chromatography (HPLC) with UV detection or Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
CPT
80307
SNOMED
385058006
Specimen volume
2-3 mL of whole blood
Stability
Room temperature (20-25°C): 7 days; Refrigerated (2-8°C): 14 days; Frozen (-20°C or below): 30 days. Avoid repeated freeze-thaw cycles.
Hemolysis max
50
Lipemia max
300
Icterus max
20
TAT
24–72 hours
Order
routine

What is this test?

The Acetazolamide Blood Level Test is a laboratory test that measures the concentration of acetazolamide in your blood. Acetazolamide is a prescription medication called a carbonic anhydrase inhibitor. This test helps your healthcare provider determine if the amount of medication in your blood is at the right level to be effective for your condition.

Why is this test performed?

This test is performed for several important reasons: (1) To monitor that acetazolamide levels are within the therapeutic range - not too low (which would make the medication ineffective) and not too high (which could cause side effects). (2) To help your doctor adjust your dosage to achieve the best treatment results. (3) To ensure patient safety by detecting potentially dangerous levels of the medication.

What conditions does acetazolamide treat?

Acetazolamide is prescribed to treat various conditions including: open-angle glaucoma (to reduce eye pressure), altitude sickness (to help your body adjust to high altitudes), certain types of epilepsy (as an anticonvulsant), and fluid retention (edema) associated with heart failure or certain medications. By measuring drug levels, doctors can ensure patients receive the optimal dose for their specific condition.

How is the test performed?

A healthcare professional will collect a blood sample from a vein in your arm using a small needle. The sample is then sent to a laboratory for analysis. The test requires a whole blood sample. Your doctor will tell you when to have this test done - often it is timed to occur just before your next dose (trough level) or at a specific time after taking your medication, depending on what information your doctor needs.

What do the results mean?

Results show the concentration of acetazolamide in your blood, measured in mass per volume. Therapeutic drug monitoring helps ensure you are receiving an appropriate dose. If levels are too low, your doctor may increase your dose. If levels are too high, your doctor may reduce your dose or check for other issues. The specific therapeutic range depends on the condition being treated and individual patient factors.

Reference range

Therapeutic range: 5-15 mcg/mL (trough levels). Peak levels (2-4 hours post-dose): 10-40 mcg/mL. Reference range may vary by laboratory methodology and clinical indication.N/A

Specimen: whole-blood

Record the exact time of last acetazolamide dose and sampling time. No fasting required. Inform laboratory of current dosing schedule for accurate interpretation. Continue medication unless otherwise instructed by prescribing physician.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Do I need to fast before this test?
Your doctor will provide specific instructions. Typically, you may be asked to have your blood drawn at a specific time relative to when you take your medication. Do not stop taking your acetazolamide unless your doctor specifically instructs you to do so.
How often will I need this test?
The frequency depends on your individual condition and treatment response. Initially, your doctor may order several tests to establish the right dose. Later, monitoring may be done periodically or if you experience symptoms or start new medications.
What are the side effects of acetazolamide?
Common side effects include increased urination, numbness or tingling in fingers or toes, taste alteration, nausea, and drowsiness. Serious side effects can include kidney stones, severe allergic reactions, and blood disorders. Contact your doctor if you experience unusual symptoms.
Will my dose be changed based on this test?
Yes, your doctor may adjust your dose based on the test results to ensure you receive the optimal amount of medication. The goal is to maintain drug levels that effectively treat your condition while minimizing side effects.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 15, 2026. · Read our editorial policy