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Understanding Your Laboratory Test: What It Is, How It Is Done, and Why You May Need It

Published: August 9, 2026Updated: August 17, 2026

This test detects the presence of acetone, a type of ketone body, in stomach fluid to help diagnose metabolic conditions.

Quick summary

{"Acetone in gastric fluid indicates the body is producing excess ketones, often due to diabetes or fasting","The test helps doctors identify diabetic ketoacidosis and other metabolic disorders","Sample is collected through gastric aspiration (stomach tube) or vomit specimen"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
40598-5
Short code
ACET-GF
Synonyms
Acetone Gast Ql
Category
chemistry
Sample
serum
Container
Sterile container (glass or plastic) for gastric fluid collection; red-top or gray-top serum tube if serum sample is collected
Units
N/A
Ref low
10
Ref high
40
Critical range
— – 500 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
Chemical reaction / Colorimetric assay
CPT
80307
SNOMED
271252004
Specimen volume
Gastric fluid: 2-5 mL preferred; Serum: 1-2 mL minimum
Stability
Room temperature: 24 hours; Refrigerated (2-8°C): 72 hours; Frozen (-20°C): 2 weeks. Avoid prolonged exposure to air.
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
24–72 hours
Order
routine

This test is a laboratory analysis that helps your healthcare provider learn more about what is happening inside your body. It may measure substances in your blood, urine, or other samples to check how well your organs are working, screen for certain conditions, or monitor treatment you are already receiving. Your provider will explain what the results mean in the context of your symptoms and overall health.

The Acetone in Gastric Fluid test detects whether acetone, a ketone body produced when the body breaks down fat for energy, is present in stomach contents. Normally, the stomach should not contain significant amounts of acetone. When detected, it typically indicates an underlying metabolic condition that requires medical attention.

Sample collection depends on the type of test your provider orders. Most commonly, a trained phlebotomist draws a small amount of blood from a vein in your arm using a thin needle, which takes only a few minutes. Some tests require a urine sample, a throat or nasal swab, or a saliva sample instead. You may receive specific instructions, such as fasting beforehand, and it is important to follow them carefully so the results are accurate.

The sample is collected through a procedure called gastric aspiration, where a thin tube is passed through the nose or mouth into the stomach to collect fluid. Alternatively, a vomit sample may be collected. The collected fluid is then sent to the laboratory for analysis.

Your healthcare provider may order this test for many reasons, including finding the cause of symptoms you are experiencing, routine screening for common conditions, monitoring a chronic illness such as diabetes or thyroid disease, or checking how well a medication is working. Early results can help detect problems before complications develop and guide decisions about your ongoing care and treatment plan.

Your doctor may order this test if you have symptoms of diabetic ketoacidosis (DKA), such as persistent vomiting, abdominal pain, rapid breathing, or confusion. It may also be used when evaluating patients with unexplained metabolic acidosis or those at risk for ketone body accumulation.

Reference range

Adult: 10-40 U/LN/A

Specimen: serum

No special preparation required. Inform the laboratory of any medications or supplements recently consumed. Provide clinical history regarding possible toxin exposure or diabetes management.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Is the test painful?
The procedure to collect gastric fluid may cause mild discomfort or a gagging sensation when the tube is passed through the throat. It is generally well-tolerated and only takes a few minutes.
How long does it take to get results?
Results are typically available within a few hours to one day, depending on the laboratory. Your healthcare provider will discuss the results with you.
What do positive results mean?
A positive result indicates the presence of acetone in your stomach contents. This usually means your body is producing too many ketones, which may be related to uncontrolled diabetes, fasting, or other metabolic conditions. Your doctor will need to perform additional tests to determine the exact cause.
Do I need to fast before this test?
Follow your healthcare provider's specific instructions. In some cases, fasting may be required to ensure accurate results, as eating can temporarily affect ketone levels.
Is this test dangerous?
The test is generally safe. The main risks are minor and include mild throat irritation, gagging, or rarely, damage to the esophagus during tube placement. These complications are uncommon when performed by trained healthcare professionals.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy