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Acetylnorfentanylurcfmmcnc

Published: August 9, 2026Updated: August 16, 2026

This test detects acetyl norfentanyl, a breakdown product of fentanyl, in urine to check for opioid use.

Quick summary

{"Acetyl norfentanyl is a substance produced when the body processes fentanyl","This test uses advanced laboratory methods to confirm fentanyl use","It is often used for pain management monitoring, addiction treatment, or drug screening programs","Results help healthcare providers understand if fentanyl or related opioids have been used","A positive result means fentanyl or its derivatives were detected in the urine"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
93471-1
Short code
AC-NF
Synonyms
Acetyl norfentanyl Ur Cfm-mCnc
Category
chemistry
Sample
urine
Container
Sterile plastic urine container (60 mL capacity)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 18 years
Method
confirm
CPT
80307
SNOMED
122465009
Specimen volume
Minimum 10 mL, preferred 20-30 mL
Stability
Room temperature: 7 days; Refrigerated (2-8°C): 30 days; Frozen (-20°C or below): 90 days
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
24–72 hours
Order
routine

What is this test?

The acetyl norfentanyl test is a specialized urine test that looks for a specific substance (acetyl norfentanyl) that your body produces when it breaks down fentanyl. Fentanyl is a powerful prescription pain medication, but it is also sometimes misused. This test uses a confirmatory method, which is a highly accurate laboratory technique to detect even small amounts of the drug.

Why is it ordered?

Healthcare providers may order this test for several reasons: to monitor patients on long-term opioid pain therapy, to check for compliance with prescribed medications, to help diagnose suspected opioid overdose, or as part of substance abuse treatment programs. It may also be used in pain management to ensure patients are taking their medications as directed.

How is the test performed?

A urine sample is collected in a clean container. The sample is then sent to a laboratory where it undergoes confirmatory testing, typically using techniques like gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS). These methods are very precise and can identify specific substances at very low levels.

What do the results mean?

A positive result indicates that fentanyl or a related opioid was recently used. A negative result means no fentanyl or its metabolites were detected. However, it is important to note that different fentanyl analogs may be detected differently, and the presence of other substances could potentially affect results. Your healthcare provider will interpret your results in the context of your medical history and other test findings.

Reference range

Not Detected (< cutoff 5 ng/mL)N/A

Specimen: urine

Collect clean-catch midstream urine specimen. Patient should avoid excessive fluid intake before collection to prevent dilution. Inform patient of any medications or supplements recently consumed.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

How long does fentanyl stay in urine?
Fentanyl and its metabolites can typically be detected in urine for 24-72 hours after use, though this varies based on factors like dosage, frequency of use, kidney function, and individual metabolism.
Will this test show if I'm taking my prescribed fentanyl?
Yes, if you are taking fentanyl as prescribed, the test should detect acetyl norfentanyl in your urine. This helps healthcare providers ensure you are taking your medication correctly.
Can this test detect other opioids?
This test is specifically designed to detect fentanyl and its metabolites. It may not detect other opioids like heroin, oxycodone, or morphine unless those substances cross-react with the test. Your healthcare provider may order additional tests if they need to check for other opioids.
Do I need to prepare for this test?
No special preparation is typically required. Simply provide a urine sample as instructed by your healthcare provider or laboratory. Inform your provider about any medications, supplements, or substances you have recently taken.
Is a positive result always due to illegal drug use?
No, a positive result could come from legitimate prescription fentanyl use. It is important to discuss your full medical history and any prescribed medications with your healthcare provider for proper interpretation.
  1. 1
    MedlinePlus - Drug TestingSource
  2. 2
    Mayo Clinic - Fentanyl (Asset)Source
  3. 3
    National Institute on Drug Abuse - FentanylSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy