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Complete Blood Count (CBC)

Published: August 9, 2026Updated: August 16, 2026

This test examines your sputum (mucus from your lungs) under a microscope to look for acid-fast bacteria, primarily the germ that causes tuberculosis.

Quick summary

{"A sputum sample is collected and examined for tuberculosis bacteria","This is the second of typically three specimens collected for testing","A positive result suggests a tuberculosis infection that requires treatment"}

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94856-2
Short code
AFB2
Synonyms
Acid fast Stn sp2 Spt Ql
Category
chemistry
Sample
sputum
Container
Sterile, leak-proof sputum collection container
Units
N/A
Ref low
10
Ref high
40
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
≥ 0 years
Method
acid-fast-stain
CPT
87206
SNOMED
122442009
Specimen volume
2-5 mL preferred
Stability
Room temperature: 2 hours; Refrigerated (2-8°C): 24-48 hours. Do not freeze.
TAT
24–72 hours
Order
routine

A complete blood count (CBC) is a common blood test that measures the cells circulating in your blood, including red blood cells, white blood cells, and platelets. It gives your health care team important information about your overall health and can help detect a wide range of conditions, from anemia and infections to blood disorders.

The acid-fast stain test is a laboratory examination that looks for acid-fast bacteria in your sputum. Acid-fast bacteria have a unique waxy coating that makes them resistant to standard staining methods. The most common acid-fast bacterium is Mycobacterium tuberculosis, which causes tuberculosis (TB). This test uses special dyes and procedures to make these bacteria visible under a microscope.

Your doctor may order a CBC as part of a routine checkup, before surgery, or if you have symptoms such as fatigue, weakness, fever, unusual bruising, or unexplained bleeding. The results help screen for conditions like anemia, infection, and blood cancers, and can also be used to monitor an ongoing illness or check how well a treatment is working.

Your healthcare provider ordered this test because you have symptoms that could indicate tuberculosis, such as a persistent cough lasting more than three weeks, coughing up blood, fever, night sweats, or unexplained weight loss. This test helps determine if you have an active TB infection in your lungs.

A health care professional will clean the skin on the inside of your elbow or the back of your hand, then use a small needle to draw a sample of blood into a tube. The process usually takes less than five minutes. Afterward, a bandage is placed on the site and the sample is sent to a laboratory for analysis. No special preparation is needed for this test.

You will need to provide a sputum sample, which is mucus that comes from deep inside your lungs when you cough. For this test (the second specimen), you will be asked to cough deeply and spit into a special sterile container. It is important to produce sputum from your lungs, not saliva. You may be asked to rinse your mouth with water before providing the sample to reduce contamination. The sample is then sent to a laboratory where it is treated with special stains and examined under a microscope.

This test is very safe and carries few risks. You may feel a quick sting when the needle goes in, and the site may be slightly sore or bruised for a day or two. Serious complications such as excessive bleeding, infection, or feeling faint are rare. If you notice ongoing bleeding, increasing pain, or swelling at the puncture site, contact your health care provider.

This test carries minimal risk. The main discomfort is the effort required to produce a proper sputum sample from the lungs. If you have difficulty producing sputum, your healthcare provider may recommend other collection methods.

Reference range

Adult: 10-40 U/LN/A

Specimen: sputum

Rinse mouth with water before collection. Collect early morning sputum specimen for optimal yield. Avoid contamination with saliva. Patient should sit upright and produce sputum from deep cough.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

Why do I need to provide multiple sputum specimens?
Providing multiple specimens (usually three, collected on separate mornings) increases the chances of detecting the bacteria if TB is present. The bacteria may not appear in every sample, so testing multiple specimens improves accuracy.
How long does it take to get results?
Results from the acid-fast stain are typically available within 24-48 hours. However, if additional culture testing is needed to confirm the diagnosis and identify the specific bacteria, results may take several weeks.
What does it mean if the test is negative?
A negative result means no acid-fast bacteria were seen in this particular sample. This could mean you do not have TB, or the bacteria are present in numbers too small to detect. If you still have symptoms, your doctor may order additional tests or collect more specimens.
Is this test painful?
No, the test is not painful. The main challenge is producing a proper sputum sample from the lungs rather than just saliva. The laboratory examination itself involves only processing your sample.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy